Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BCAP31 / BAP31 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125800-100
Catalog Number: LS-G125800
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAP31(BCAP31) (NM_001139457), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 6C6-AG | BCR-associated protein 31 | BAP31 | BCAP31 | BCR-associated protein Bap31 | DXS1357E | p28 Bap31 | CDM | p28 | Protein CDM
- Origin Species
- Human
- NCBI GenBank
- NM_005745
- SwissProt
- P51572
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAEASSSWC PGTALPEERL SVKRASEISG FLGQGSSGEA ALDVLTHVLE GAGNKLTSSC61GKPSSNRMSL QWTAVATFLY AEVFVVLLLC IPFISPKRWQ KIFKSRLVEL LVSYGNTFFV121VLIVILVLLV IDAVREIRKY DDVTEKVNLQ NNPGAMEHFH MKLFRAQRNL YIAGFSLLLS181FLLRRLVTLI SQQATLLASN EAFKKQAESA SEAAKKYMEE NDQLKKGAAV DGGKLDVGNA241EVKLEEENRS LKADLQKLKD ELASTKQKLE KAENQVLAMR KQSEGLTKEY DRLLEEHAKL301QAAVDGPMDK KEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
313 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.