Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BCAP / PHF11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120327-100
Catalog Number: LS-G120327
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF11 (NM_001040443), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BCAP | IGER | IGHER | IGEL | PHD finger protein 11 | NY-REN-34 | PHF11 | APY | RP11-185C18.3 | IgE responsiveness (atopic) | NY-REN-34 antigen | NYREN34
- Origin Species
- Human
- NCBI GenBank
- NM_001040443
- SwissProt
- Q9UIL8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKRTCALCP KDVEYNVLYF AQSENIAAHE NCLLYSSGLV ECEDQDPLNP DRSFDVESVK61KEIQRGRKLK CKFCHKRGAT VGCDLKNCNK NYHFFCAKKD DAVPQSDGVR GIYKLLCQQH121AQFPIIAQSA KFSGVKRKRG RKKPLSGNHV QPPETMKCNT FIRQVKEEHG RHTDATVKVP181FLKKCKEAGL LNYLLEEILD KVHSIPEKLM DETTSESDYE EIGSALFDCR LFEDTFVNFQ241AAIEKKIHAS QQRWQQLKEE IELLQDLKQT LCSFQENRDL MSSSTSISSL SYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
292 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.