LSBio
BBX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BBX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BBX antibodies →
$725.00each
Catalog Number: LS-G126015-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BBX (NM_001142568), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bobby sox homolog | BBX | HSPC339 | HMG box-containing protein 2 | MDS001 | X 001 protein | Bobby sox homolog (Drosophila) | HBP2
Origin Species
Human
NCBI GenBank
NM_020235
SwissProt
Q8WY36

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKGSNRNKDH SAEGEGVGKR PKRKCLQWHP LLAKKLLDFS EEEEEEDEEE DIDKVQLLGA
61DGLEQDVGET EDDESPEQRA RRPMNAFLLF CKRHRSLVRQ EHPRLDNRGA TKILADWWAV
121LDPKEKQKYT DMAKEYKDAF MKANPGYKWC PTTNKPVKSP TPTVNPRKKL WAFPSDSSRD
181LPSPKKAKTE EMPQLNFGMA DPTQMGGLSM LLLAGEHALG TPEVSSGTCR PDVSESPELR
241QKSPLFQFAE ISSSTSHSDA STKQCQTSAL FQFAEISSNT SQLGGAEPVK RCGKSALFQL
301AEMCLASEGM KMEESKLIKA KESDGGRIKE LEKGKEEKEI KMEKTDETRL QKEAEFEKSA
361KENLRDSKEL RNFEALQIDD IMAIKMEDPK EIRKEELEED HKCSHFPDFS YSASSKIIIS
421DVPSRKDHMC HPHGIMIIED PAALNKPEKL KKKKKKSKMD RHGNDKSTPK KTCKKRQSSE
481SDIESVIYTI EAVAKGDWGI EKLGDTPRKK VRTSSSGKGS ILDAKPPKKK VKSREKKMSK
541EKSSDTTKES RPPDFISISA SKNISGETPE GIKAEPLTPM EDALPPSLSG QAKPEDSDCH
601RKIETCGSRK SERSCKGALY KTLVSEGMLT SLRANVDRGK RSSGKGNSSD HEGCWNEESW
661TFSQSGTSGS KKFKKTKPKE DCLLGSAKLD EEFEKKFNSL PQYSPVTFDR KCVPVPRKKK
721KTGNVSSEPT KTSKGPFQSQ KKNLFHKIVS KYKHKKEKPN VPEKGSGDKW SNKQLFLDAI
781HPTEAIFSED RNTMEPVHKV KNIPSIFNTP EPTTTQEPLV GSQKRKARKT KITHLVRTAD
841GRVSPAGGTL DDKPKEQLQR SLPKATETDC NDKCSHNTEV GETRSSTPEM PAVSAFFSLA
901ALAEVAAMEN VHRGQRSTPL THDGQPKEMP QAPVLISCAD Q
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

941 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.