Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Basigin / Emmprin / CD147 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102578-100
Catalog Number: LS-G102578
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD147(BSG) (NM_198589), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5F7 | Basigin | Basigin (Ok blood group) | CD147 antigen | BSG | EMMPRIN | OK | OK blood group antigen | TCSF | CD147 | M6
- Origin Species
- Human
- NCBI GenBank
- NM_001728
- SwissProt
- P35613
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 27.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAALFVLLG FALLGTHGAS GAAGTVFTTV EDLGSKILLT CSLNDSATEV TGHRWLKGGV61VLKEDALPGQ KTEFKVDSDD QWGEYSCVFL PEPMGTANIQ LHGPPRVKAV KSSEHINEGE121TAMLVCKSES VPPVTDWAWY KITDSEDKAL MNGSESRFFV SSSQGRSELH IENLNMEADP181GQYRCNGTSS KGSDQAIITL RVRSHLAALW PFLGIVAEVL VLVTIIFIYE KRRKPEDVLD241DDDAGSAPLK SSGQHQNDKG KNVRQRNSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
269 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.