Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BAP29 / BCAP29 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121929-100
Catalog Number: LS-G121929
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAP29(BCAP29) (NM_001008405), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BAP29 | BCAP29 | BCR-associated protein 29
- Origin Species
- Human
- NCBI GenBank
- NM_001008405
- SwissProt
- Q9UHQ4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLQWAAVAT FLYAEIGLIL IFCLPFIPPQ RWQKIFSFNV WGKIATFWNK AFLTIIILLI61VLFLDAVREV RKYSSVHTIE KSSTSRPDAY EHTQMKLFRS QRNLYISGFS LFFWLVLRRL121VTLITQLAKE LSNKGVLKTQ AENTNKAAKK FMEENEKLKR ILKSHGKDEE CVLEAENKKL181VEDQEKLKTE LRKTSDALSK AQNDVMEMKM QSERLSKEYD QLLKEHSELQ HSSFGEFLSK241RSHKNGSIGK QTGSRKGSFR KRQQEKTVNF IKDTCNILCQ NDNFVMLASR KFKFRKMHYD301RFVIFLMPHI GCIVMALSKY LMMFQIYCKV CIPALKKNIS MLNTIFTYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
348 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.