LSBio
BANP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BANP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129953-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BANP (NM_001173543), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BEND1 | SMARBP1 | SMAR1 | BANP | Protein BANP
Origin Species
Human
NCBI GenBank
NM_017869
SwissProt
Q8N9N5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMSEHDLADV VQIAVEDLSP DHPVVLENHV VTDEDEPALK RQRLEINCQD PSIKTICLRL
61DSIEAKLQAL EATCKSLEEK LDLVTNKQHS PIQVPMVAGS PLGATQTCNK VRCVVPQTTV
121ILNNDRQNAI VAKMEDPLSN RAPDSLENVI SNAVPGRRQN TIVVKVPGQE DSHHEDGESG
181SEASDSVSSC GQAGSQSIGS NVTLITLNSE EDYPNGTWLG DENNPEMRVR CAIIPSDMLH
241ISTNCRTAEK MALTLLDYLF HREVQAVSNL SGQGKHGKKQ LDPLTIYGIR CHLFYKFGIT
301ESDWYRIKQS IDSKCRTAWR RKQRGQSLAV KSFSRRTPNS SSYCPSEPMM STPPPASELP
361QPQPQPQALH YALANAQQVQ IHQIGEDGQV QVGHLHIAQV PQGEQVQITQ DSEGNLQIHH
421VGQDGQLLEA TRIPCLLAPS VFKASSGQVL QGAQLIAVAS SDPAAAGVDG SPLQGSDIQV
481QYVQLAPVSD HTAGAQTAEA LQPTLQPEMQ LEHGAIQIQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

519 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.