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BAIAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BAIAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117000-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAIAP3 (NM_003933), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BAIAP3 | BAI-associated protein 3 | BAI1-associated protein 3 | BAP3 | KIAA0734
Origin Species
Human
NCBI GenBank
NM_003933
SwissProt
O94812

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRPRGAAFAA GPPGDLHLGT AIGFAGAIWR SRSPAMSTLL DIKSSVLRQV QVCPSFRRRT
61EQDPGSASAD PQEPATGAWK PGDGVEFFAH MRLMLKKGEG RQGLPCLEVP LRSGSPAPPE
121PVDPSLGLRA LAPEEVEMLY EEALYTVLYR AGTMGPDQVD DEEALLSYLQ QVFGTSLEEH
181TEAIERVRKA KAPTYALKVS VMRAKNLLAK DPNGFSDPYC MLGILPASDA TREPRAQKEQ
241RFGFRKGSKR GGPLPAKCIQ VTEVKSSTLN PVWKEHFLFE IEDVSTDQLH LDIWDHDDDV
301SLVEACRKLN EVIGLKGMGR YFKQIVKSAR ANGTAGPTED HTDDFLGCLN IPVREVPVAG
361VDRWFKLEPR SSASRVQGHC HLVLKLITTQ RDTAMSQRGR SGFLSHLLLL SHLLRLEHSA
421EEPNSSSWRG ELSTPAATIL CLHGAQSNLS PLQLAVLHWQ VSSRHHQTCT LDYSYLLGLL
481EDMQAHWEEA PSLPQEQEES LADSLSAFSE FGLQLLRQLR DYFPATNSTA VHRLELLLKC
541LGKLQLFQPS FEICPFESEL NMDIAAALKR GNREWYDRIL NDKSPREQPG PQRLPGLVVL
601ADAVYDDLQF CYSVYASLFH SILNVDVFTL TFRQLERLVA EEAWVLTEEL SPKMTLEVAS
661GLFELYLTLA DLQRFWDSIP GRDSRSLALA GIHAPFLPAV KLWFQVLRDQ AKWRLQGAVD
721MDTLEPVDAS SRHSSSAATA GLCLSHIQEL WVRLAWPDPA QAQGLGTQLG QDVCEATLFY
781TELLRKKVDT QPGAAGEAVS EALCVVLNNV ELVRKAAGQA LKGLAWPEGA TGPEGVLPRP
841LLSCTQALDD DLQREAHTVT AHLTSKMVGD IRKYVQHISL SPDSIQNDEA VAPLMKYLDE
901KLALLNASLV KGNLSRVLEA LWELLLQAIL QALGANRDVS ADFYSRFHFT LEALVSFFHA
961EGQGLPLESL RDGSYKRLKE ELRLHKCSTR ECIEQFYLDK LKQRTLEQNR FGRLSVRCHY
1021EAAEQRLAVE VLHAADLLPL DANGLSDPFV IVELGPPHLF PLVRSQRTQV KTRTLHPVYD
1081ELFYFSVPAE ACRRRAACVL FTVMDHDWLS TNDFAGEAAL GLGGVTGVAR PQVGGGARAG
1141QPVTLHLCRP RAQVRSALRR LEGRTSKEAQ EFVKKLKELE KCMEADP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1187 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.