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BAHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BAHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BAHD1 antibodies →
$504.00each
Catalog Number: LS-G113587-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAHD1 (NM_014952), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0945 | BAHD1
Origin Species
Human
NCBI GenBank
NM_014952
SwissProt
Q8TBE0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTHTRRKSLP MLSSGLTGRR EPLQMEDSNM EQGVEGVEPG MPESPGHLTG RRKNYPLRKR
61PLVPEKPKAC KVLLTRLENV AGPRSADEAD ELPPDLPKPP SPAPSSEDPG LAQPRKRRLA
121SLNAEALNNL LLEREDTSSL AGTRRSRAGD PHRSRDRDRA TGGWSSSKKR PRLGDLGGGS
181RHLSPEPAPD EGPRRDGDPA PKRLASLNAA AFLKLSQERE LPLRLPRAHA EVDGRSTEPP
241APKAPRPKWP KVNGKNYPKA WQGASSGEAA GPPGWQGCPD EPWPSATPCG PSVQPSHKPL
301SKALESPLGL RPHLPLLMGG QAALKPEPGR PGEESPAPKQ ELHQPSFPTP QLSPLPMPGN
361PADYNGLCVG PELTALGSFY LYCGQEGLQC GGYSPCPMLP EGKLSPVAAP HEEGLLLAPS
421SVPSGTPFQH PPWGSSRYCS SEDTGVNGYS ICGVLPLSVT HAGTTCGGCP YKMPFAAEGC
481RSLGQLEFPL PEAGHPASPA HPLLGCPVPS VPPAAEPVPH LQTPTSEPQT VARACPQSAK
541PPSGSKSGLR TGSSCRHTAR SKAARRPSHP KQPRVQRPRP RRRRRRRTNG WVPVGAACEK
601AVYVLDEPEP AIRKSYQAVE RHGETIRVRD TVLLKSGPRK TSTPYVAKIS ALWENPESGE
661LMMSLLWYYR PEHLQGGRSP SMHEPLQNEV FASRHQDQNS VACIEEKCYV LTFAEYCRFC
721AMAKRRGEGL PSRKTALVPP SADYSTPPHR TVPEDTDPEL VFLCRHVYDF RHGRILKNPQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

780 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.