Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BAG6 / G3 / Scythe Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107819-100
Catalog Number: LS-G107819
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAT3(BAG6) (NM_080702), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BAG6 | BCL2-associated athanogene 6 | D6S52E | HLA-B associated transcript 3 | HLA-B-associated transcript 3 | HLA-B associated transcript-3 | G3 | Protein G3 | Protein Scythe | Scythe | BAG-6 | BAT3
- Origin Species
- Human
- NCBI GenBank
- NM_004639
- SwissProt
- P46379
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 118.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPNDSTSTA VEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV SIPSEKQRLI61YQGRVLQDDK KLQEYNVGGK VIHLVERAPP QTHLPSGASS GTGSASATHG GGSPPGTRGP121GASVHDRNAN SYVMVGTFNL PSDGSAVDVH INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL181SRMECRGGPQ PQHSQPPPQP PAVTPEPVAL SSQTSEPVES EAPPREPMEA EEVEERAPAQ241NPELTPGPAP AGPTPAPETN APNHPSPAEY VEVLQELQRL ESRLQPFLQR YYEVLGAAAT301TDYNNNHEGR EEDQRLINLV GESLRLLGNT FVALSDLRCN LACTPPRHLH VVRPMSHYTT361PMVLQQAAIP IQINVGTTVT MTGNGTRPPP TPNAEAPPPG PGQASSVAPS STNVESSAEG421APPPGPAPPP ATSHPRVIRI SHQSVEPVVM MHMNIQDSGT QPGGVPSAPT GPLGPPGHGQ481TLGQQVPGFP TAPTRVVIAR PTPPQARPSH PGGPPVSGTL QGAGLGTNAS LAQMVSGLVG541QLLMQPVLVA QGTPGMAPPP APATASASAG TTNTATTAGP APGGPAQPPP TPQPSMADLQ601FSQLLGNLLG PAGPGAGGPG VASPTITVAM PGVPAFLQGM TDFLQATQTA PPPPPPPPPP661PPAPEQQTMP PPGSPSGGAG SPGGLGLESL SPEFFTSVVQ GVLSSLLGSL GARAGSSESI721AAFIQRLSGS SNIFEPGADG ALGFFGALLS LLCQNFSMVD VVMLLHGHFQ PLQRLQPQLR781SFFHQHYLGG QEPTPSNIRM ATHTLITGLE EYVRESFSLV QVQPGVDIIR TNLEFLQEQF841NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG QQMELAAVIN GRIRRMSRGV901NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI LRYVRRVGDP PQPLPEEPME VQGAERASPE961PQRENASPAP GTTAEEAMSR GPPPAPEGGS RDEQDGASAE TEPWAAAVPP EWVPIIQQDI1021QSQRKVKPQP PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA VSRAAKAAGA RPLTSPESLS1081RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAQR AFADDPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1126 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.