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BAG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BAG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121626-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BAG5 (NM_001015048), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BAG-5 | KIAA0873 | BAG5 | Bcl-2-associated athanogene 5 | BCL2-associated athanogene 5
Origin Species
Human
NCBI GenBank
NM_004873
SwissProt
Q9UL15

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.03 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRFHWLPTLS EPFDRNQELE TCIRPLWTPS GSACETEHNK SMDMGNQHPS ISRLQEIQKE
61VKSVEQQVIG FSGLSDDKNY KKLERILTKQ LFEIDSVDTE GKGDIQQARK RAAQETERLL
121KELEQNANHP HRIEIQNIFE EAQSLVREKI VPFYNGGNCV TDEFEEGIQD IILRLTHVKT
181GGKISLRKAR YHTLTKICAV QEIIEDCMKK QPSLPLSEDA HPSVAKINFV MCEVNKARGV
241LIALLMGVNN NETCRHLSCV LSGLIADLDA LDVCGRTEIR NYRREVVEDI NKLLKYLDLE
301EEADTTKAFD LRQNHSILKI EKVLKRMREI KNELLQAQNP SELYLSSKTE LQGLIGQLDE
361VSLEKNPCIR EARRRAVIEV QTLITYIDLK EALEKRKLFA CEEHPSHKAV WNVLGNLSEI
421QGEVLSFDGN RTDKNYIRLE ELLTKQLLAL DAVDPQGEEK CKAARKQAVR LAQNILSYLD
481LKSDEWEY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

488 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.