Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
B3GNT3 / TMEM3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114068-100
Catalog Number: LS-G114068
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human B3GNT3 (NM_014256), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B3GALT8 | B3GNT-3 | Beta-3-Gx-T8 | Beta3Gn-T3 | BGnT-3 | B3GAL-T8 | Beta3Gal-T8 | Beta3GalT8 | B3GN-T3 | Beta-1 | 3-GalTase 8 | 3-Gn-T3 | Core1-beta3GlcNAcT | HP10328 | Transmembrane protein 3 | B3GNT3 | TMEM3
- Origin Species
- Human
- NCBI GenBank
- NM_014256
- SwissProt
- Q9Y2A9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKYLRHRRPN ATLILAIGAF TLLLFSLLVS PPTCKVQEQP PAIPEALAWP TPPTRPAPAP61CHANTSMVTH PDFATQPQHV QNFLLYRHCR HFPLLQDVPP SKCAQPVFLL LVIKSSPSNY121VRRELLRRTW GRERKVRGLQ LRLLFLVGTA SNPHEARKVN RLLELEAQTH GDILQWDFHD181SFFNLTLKQV LFLQWQETRC ANASFVLNGD DDVFAHTDNM VFYLQDHDPG RHLFVGQLIQ241NVGPIRAFWS KYYVPEVVTQ NERYPPYCGG GGFLLSRFTA AALRRAAHVL DIFPIDDVFL301GMCLELEGLK PASHSGIRTS GVRAPSQHLS SFDPCFYRDL LLVHRFLPYE MLLMRDALNQ361PNLTCGNQTQ IYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
372 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.