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B3GALNT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

B3GALNT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all B3GALNT2 antibodies →
$504.00each
Catalog Number: LS-G106222-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human B3GALNT2 (NM_152490), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B3GalNAc-T2 | B3GALNT2 | Beta-1 | 3-GalNAc-T2
Origin Species
Human
SwissProt
Q8NCR0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRNWLVLLCP CVLGAALHLW LRLRSPPPAC ASGAGPADQL ALFPQWKSTH YDVVVGVLSA
61RNNHELRNVI RSTWMRHLLQ HPTLSQRVLV KFIIGAHGCE VPVEDREDPY SCKLLNITNP
121VLNQEIEAFS LSEDTSSGLP EDRVVSVSFR VLYPIVITSL GVFYDANDVG FQRNITVKLY
181QAEQEEALFI ARFSPPSCGV QVNKLWYKPV EQFILPESFE GTIVWESQDL HGLVSRNLHK
241VTVNDGGGVL RVITAGEGAL PHEFLEGVEG VAGGFIYTIQ EGDALLHNLH SRPQRLIDHI
301RNLHEEDALL KEESSIYDDI VFVDVVDTYR NVPAKLLNFY RWTVETTSFN LLLKTDDDCY
361IDLEAVFNRI VQKNLDGPNF WWGNFRLNWA VDRTGKWQEL EYPSPAYPAF ACGSGYVISK
421DIVKWLASNS GRLKTYQGED VSMGIWMAAI GPKRYQDSLW LCEKTCETGM LSSPQYSPWE
481LTELWKLKER CGDPCRCQAR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

500 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.