Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
B1R / BDKRB1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99123-100
Catalog Number: LS-G99123
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BDKRB1 (NM_000710), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BKB1R | Bradykinin receptor 1 | B1BKR | B1R | BDKRB1 | Bk b1 receptor | BK-1 receptor | BRADYB1 | Bradykinin receptor B1 | Kinin b1 receptor | B1 bradykinin receptor | BKR1 | Bradykinin B1 receptor
- NCBI GenBank
- NM_000710
- SwissProt
- P46663
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSWPPLEL QSSNQSQLFP QNATACDNAP EAWDLLHRVL PTFIISICFF GLLGNLFVLL61VFLLPRRQLN VAEIYLANLA ASDLVFVLGL PFWAENIWNQ FNWPFGALLC RVINGVIKAN121LFISIFLVVA ISQDRYRVLV HPMASRRQQR RRQARVTCVL IWVVGGLLSI PTFLLRSIQA181VPDLNITACI LLLPHEAWHF ARIVELNILG FLLPLAAIVF FNYHILASLR TREEVSRTRC241GGRKDSKTTA LILTLVVAFL VCWAPYHFFA FLEFLFQVQA VRGCFWEDFI DLGLQLANFF301AFTNSSLNPV IYVFVGRLFR TKVWELYKQC TPKSLAPISS SHRKEIFQLF WRNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
353 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.