Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AZI2 / NAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110363-100
Catalog Number: LS-G110363
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AZI2 (NM_022461), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-azacytidine induced 2 | 5-azacytidine induced gene 2 | AZI2 | Nak-associated protein 1 | NAP1 | TILP | AZ2
- Origin Species
- Human
- NCBI GenBank
- NM_022461
- SwissProt
- Q9H6S1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDALVEDDIC ILNHEKAHKR DTVTPVSIYS GDESVASHFA LVTAYEDIKK RLKDSEKENS61LLKKRIRFLE EKLIARFEEE TSSVGREQVN KAYHAYREVC IDRDNLKSKL DKMNKDNSES121LKVLNEQLQS KEVELLQLRT EVETQQVMRN LNPPSSNWEV EKLSCDLKIH GLEQELELMR181KECSDLKIEL QKAKQTDPYQ EDNLKSRDLQ KLSISSDNMQ HAYWELKREM SNLHLVTQVQ241AELLRKLKTS TAIKKACAPV GCSEDLGRDS TKLHLMNFTA TYTRHPPLLP NGKALCHTTS301SPLPGDVKVL SEKAILQSWT DNERSIPNDG TCFQEHSSYG RNSLEDNSWV FPSPPKSSET361AFGETKTKTL PLPNLPPLHY LDQHNQNCLY KNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.