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AVPR1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AVPR1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G99121-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Vasopressin V1b receptor(AVPR1B) (NM_000707), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AVPR1B | AVPR3 | V1bR | AVPR V1b | AVPR V3 | V1b receptor | V1b vasopressin receptor | Vasopressin V1b receptor | Vasopressin V3 receptor | VPR3
NCBI GenBank
AF030512
SwissProt
P47901

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSGPLWDAN PTPRGTLSAP NATTPWLGRD EELAKVEIGV LATVLVLATG GNLAVLLTLG
61QLGRKRSRMH LFVLHLALTD LAVALFQVLP QLLWDITYRF QGPDLLCRAV KYLQVLSMFA
121STYMLLAMTL DRYLAVCHPL RSLQQPGQST YLLIAAPWLL AAIFSLPQVF IFSLREVIQG
181SGVLDCWADF GFPWGPRAYL TWTTLAIFVP PVTMLTACYS LICHEICKNL KVKTQAWRVG
241GGGWRTWDRP SPSTLAATTR GLPSRVSSIN TISRAKIRTV KMTFVIVLAY IACWAPFFSV
301QMWSVWDKNV PDEDSTNVAF TISMLLGNLN SCCNPWIYMG FNSHLLPRPL RHLACCGGPQ
361PRMRRRLSDG SLSSRHTTLL TRSSCPATLS LSLSLTLSGR PRPEESPRDL ELADGEGTAE
421TIIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

424 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.