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AVL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AVL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AVL9 antibodies →
$725.00each
Catalog Number: LS-G113495-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AVL9 (NM_015060), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AVL9 | KIAA0241 | AVL9 homolog (S. cerevisiase)
Origin Species
Human
NCBI GenBank
D87682
SwissProt
Q8NBF6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKARRGGDG VPRGPVLHIV VVGFHHKKGC QVEFSYPPLI PGDGHDSHTL PEEWKYLPFL
61ALPDGAHNYQ EDTVFFHLPP RNGNGATVFG ISCYRQIEAK ALKVRQADIT RETVQKSVCV
121LSKLPLYGLL QAKLQLITHA YFEEKDFSQI SILKELYEHM NSSLGGASLE GSQVYLGLSP
181RDLVLHFRHK VLILFKLILL EKKVLFYISP VNKLVGALMT VLSLFPGMIE HGLSDCSQYR
241PRKSMSEDGG LQESNPCADD FVSASTADVS HTNLGTIRKV MAGNHGEDAA MKTEEPLFQV
301EDSSKGQEPN DTNQYLKPPS RPSPDSSESD WETLDPSVLE DPNLKEREQL GSDQTNLFPK
361DSVPSESLPI TVQPQANTGQ VVLIPGLISG LEEDQYGMPL AIFTKGYLCL PYMALQQHHL
421LSDVTVRGFV AGATNILFRQ QKHLSDAIVE VEEALIQIHD PELRKLLNPT TADLRFADYL
481VRHVTENRDD VFLDGTGWEG GDEWIRAQFA VYIHALLAAT LQLDNEKILS DYGTTFVTAW
541KNTHNYRVWN SNKHPALAEI NPNHPFQGQY SVSDMKLRFS HSVQNSERGK KIGNVMVTTS
601RNVVQTGKAV GQSVGGAFSS AKTAMSSWLS TFTTSTSQSL TEPPDEKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

648 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.