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AUTS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AUTS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AUTS2 antibodies →
$725.00each
Catalog Number: LS-G124582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AUTS2 (NM_001127231), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Autism-related protein 1 | AUTS2 | FBRSL2 | KIAA0442
Origin Species
Human
SwissProt
Q8WXX7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDGPTRGHGL RKKRRSRSQR DRERRSRGGL GAGAAGGGGA GRTRALSLAS SSGSDKEDNG
61KPPSSAPSRP RPPRRKRRES TSAEEDIIDG FAMTSFVTFE ALEKDVALKP QERVEKRQTP
121LTKKKREALT NGLSFHSKKS RLSHPHHYSS DRENDRNLCQ HLGKRKKMPK ALRQLKPGQN
181SCRDSDSESA SGESKGFHRS SSRERLSDSS APSSLGTGYF CDSDSDQEEK ASDASSEKLF
241NTVIVNKDPE LGVGTLPEHD SQDAGPIVPK ISGLERSQEK SQDCCKEPIF EPVVLKDPCP
301QVAQPIPQPQ TEPQLRAPSP DPDLVQRTEA PPQPPPLSTQ PPQGPPEAQL QPAPQPQVQR
361PPRPQSPTQL LHQNLPPVQA HPSAQSLSQP LSAYNSSSLS LNSLSSSRSS TPAKTQPAPP
421HISHHPSASP FPLSLPNHSP LHSFTPTLQP PAHSHHPNMF APPTALPPPP PLTSGSLQVA
481GHPAGSTYSE QDILRQELNT RFLASQSADR GASLGPPPYL RTEFHQHQHQ HQHTHQHTHQ
541HTFTPFPHAI PPTAIMPTPA PPMFDKYPTK VDPFYRHSLF HSYPPAVSGI PPMIPPTGPF
601GSLQGAFQPK LTDPFRPMLR KPGKWCAMHV HIAWQIYHHQ QKVKKQMQSD PHKLDFGLKP
661EFLSRPPGPS LFGAIHHPHD LARPSTLFSA AGAAHPTGTP FGPPPHHSNF LNPAAHLEPF
721NRPSTFTGLA AVGGNAFGGL GNPSVTPNSM FGHKDGPSVQ NFSNPHEPWN RLHRTPPSFP
781TPPPWLKPGE LERSASAAAH DRDRDVDKRD SSVSKDDKER ESVEKRHSSH PSPAPVLPVN
841ALGHTRSSTE QIRAHLNTEA REKDKPKERE RDHSESRKDL AADEHKAKEG HLPEKDGHGH
901EGRAAGEEAK QLARVPSPYV RTPVVESARP NSTSSREAEP RKGEPAYENP KKSSEVKVKE
961ERKEDHDLPP EAPQTHRASE PPPPNSSSSV HPGPLASMPM TVGVTGIHPM NSISSLDRTR
1021MMTPFMGISP LPGGERFPYP SFHWDPIRDP LRDPYRELDI HRRDPLGRDF LLRNDPLHRL
1081STPRLYEADR SFRDREPHDY SHHHHHHHHP LSVDPRREHE RGGHLDERER LHMLREDYEH
1141TRLHSVHPAS LDGHLPHPSL ITPGLPSMHY PRISPTAGNQ NGLLNKTPPT AALSAPPPLI
1201STLGGRPVSP RRTTPLSAEI RERPPSHTLK DIEAR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1235 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.