Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AURKA / Aurora-A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103693-100
Catalog Number: LS-G103693
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Aurora A(AURKA) (NM_198437), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIK | AIRK1 | ARK1 | Aurora-A kinase | AURKA | Aurora kinase A | Aurora/IPL1-like kinase | AYK1 | AURA | AURORA2 | Breast tumor-amplified kinase | Breast-tumor-amplified kinase | BTAK | HARK1 | IPL1-related kinase | Serine/threonine kinase 6 | STK7 | STK6 | PPP1R47 | ARK-1 | Aurora 2 | Aurora-A | Aurora-related kinase 1 | Aurora/IPL1-related kinase 1 | IAK1 | Serine/threonine kinase 15 | STK15
- Origin Species
- Human
- NCBI GenBank
- NM_003600
- SwissProt
- O14965
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRVPLQ61AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN121EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILA LKVLFKAQLE KAGVEHQLRR181EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL241ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM301IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD FVTEGARDLI361SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
403 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.