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ATXN7L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATXN7L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ATXN7L2 antibodies →
$504.00each
Catalog Number: LS-G105787-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATXN7L2 (NM_153340), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ataxin 7-like 1 | Ataxin 7-like 2 | Ataxin-7-like protein 2 | ATXN7L2
Origin Species
Human
SwissProt
Q5T6C5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVRERAAAA MAALERRVPS LDDFAGQSWS SWVERADLPA ADGAELEESS KNTKKLDAMT
61LIKEDMSIFG HCPAHDDFYL VVCNHCSQVV KPQAFQKHCE RRHGPLSKLY GRAPPPPPAP
121ASSQKCHVVN GQGPACRAPG STKTSSREKG QGSRSRGHQP PEKTQKDNLC QPGGLTKDSP
181GKPPMAPPSK EPPGRENIEI IPSEGSSHWA EGSPPEKEPS GTRLPPKTHR KMARKECDLN
241RQCGVINPET KKICTRLLTC KIHSVHQRRE VQGRAKDFDV LVAELKANSR KGESPKEKSP
301GRKEQVLERP SQELPSSVQV VAAVAAPSST FSVRAKQTYP YCALPRSRAS SESELDDEGP
361CGGDGDPGLF PFPMPRGGTQ ASSEESEEEG TSDDLHPPPD CHYATRPPRP QAFCTFGSRL
421VSPGCYVFSR RLDRFCSALS SMLERHLSTH MWKKIPPAAE PPVHLVNSPL SAPLSPSSTG
481TCPRLPGPTL RPACPASMPP TKDNLVPSYP AGSPSVAAAC SQAECMGEPG YHLTTACQHA
541IPVLQQAAAF QGQQVIQRQG RGGGGGPFSK AEVIPWPYHS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

580 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.