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ATXN2L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATXN2L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106461-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Ataxin 2 like(ATXN2L) (NM_148414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A2D | A2LP | A2RP | Ataxin 2 related protein | Ataxin-2 domain protein | Ataxin-2-related protein | ATXN2L | A2LG | Ataxin 2-like | Ataxin-2-like protein
Origin Species
Human
NCBI GenBank
NM_007245
SwissProt
Q8WWM7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLKPQPLQQP SQPQQPPPTQ QAVARRPPGG TSPPNGGLPG PLATSAAPPG PPAAASPCLG
61PVAAAGSGLR RGAEGILAPQ PPPPQQHQER PGAAAIGSAR GQSTGKGPPQ SPVFEGVYNN
121SRMLHFLTAV VGSTCDVKVK NGTTYEGIFK TLSSKFELAV DAVHRKASEP AGGPRREDIV
181DTMVFKPSDV MLVHFRNVDF NYATKDKFTD SAIAMNSKVN GEHKEKVLQR WEGGDSNSDD
241YDLESDMSNG WDPNEMFKFN EENYGVKTTY DSSLSSYTVP LEKDNSEEFR QRELRAAQLA
301REIESSPQYR LRIAMENDDG RTEEEKHSAV QRQGSGRESP SLASREGKYI PLPQRVREGP
361RGGVRCSSSR GGRPGLSSLP PRGPHHLDNS SPGPGSEARG INGGPSRMSP KAQRPLRGAK
421TLSSPSNRPS GETSVPPPPA VGRMYPPRSP KSAAPAPISA SCPEPPIGSA VPTSSASIPV
481TSSVSDPGVG SISPASPKIS LAPTDVKELS TKEPGRTLEP QELARIAGKV PGLQNEQKRF
541QLEELRKFGA QFKLQPSSSP ENSLDPFPPR ILKEEPKGKE KEVDGLLTSE PMGSPVSSKT
601ESVSDKEDKP PLAPSGGTEG PEQPPPPCPS QTGSPPVGLI KGEDKDEGPV AEQVKKSTLN
661PNAKEFNPTK PLLSVNKSTS TPTSPGPRTH STPSIPVLTA GQSGLYSPQY ISYIPQIHMG
721PAVQAPQMYP YPVSNSVPGQ QGKYRGAKGS LPPQRSDQHQ PASAPPMMQA AAAAGPPLVA
781ATPYSSYIPY NPQQFPGQPA MMQPMAHYPS QPVFAPMLQS NPRMLTSGSH PQAIVSSSTP
841QYPSAEQPTP QALYATVHQS YPHHATQLHA HQPQPATTPT GSQPQSQHAA PSPVQHQAGQ
901APHLGSGQPQ QNLYHPGALT GTPPSLPPGP SAQSPQSSFP QPAAVYAIHH QQLPHGFTNM
961AHVTQAHVQT GITAAPPPHP GAPHPPQVML LHPPQSHGGP PQGAVPQSGV PALSASTPSP
1021YPYIGHPQGE QPGQAPGFPG GADDRILCRV GRSHSRRRQG LAPGSVLCFP PSSLSCDPAA
1081PLPTASPALS DPDCLLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1097 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.