Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ATRN / Attractin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107024-100
Catalog Number: LS-G107024
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Attractin(ATRN) (NM_139322), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATRN | Attractin-2 | Attractin | DPPT-L | KIAA0548 | Mahogany protein | MGCA | Mahogany homolog
- Origin Species
- Human
- SwissProt
- O75882
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 141.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVAAAAATEA RLRRRTAATA ALAGRSGGPH WDWDVTRAGR PGLGAGLRLP RLLSPPLRPR61LLLLLLLLSP PLLLLLLPCE AEAAAAAAAV SGSAAAEAKE CDRPCVNGGR CNPGTGQCVC121PAGWVGEQCQ HCGGRFRLTG SSGFVTDGPG NYKYKTKCTW LIEGQPNRIM RLRFNHFATE181CSWDHLYVYD GDSIYAPLVA AFSGLIVPER DGNETVPEVV ATSGYALLHF FSDAAYNLTG241FNITYSFDMC PNNCSGRGEC KISNSSDTVE CECSENWKGE ACDIPHCTDN CGFPHRGICN301SSDVRGCSCF SDWQGPGCSV PVPANQSFWT REEYSNLKLP RASHKAVVNG NIMWVVGGYM361FNHSDYNMVL AYDLASREWL PLNRSVNNVV VRYGHSLALY KDKIYMYGGK IDSTGNVTNE421LRVFHIHNES WVLLTPKAKE QYAVVGHSAH IVTLKNGRVV MLVIFGHCPL YGYISNVQEY481DLDKNTWSIL HTQGALVQGG YGHSSVYDHR TRALYVHGGY KAFSANKYRL ADDLYRYDVD541TQMWTILKDS RFFRYLHTAV IVSGTMLVFG GNTHNDTSMS HGAKCFSSDF MAYDIACDRW601SVLPRPDLHH DVNRFGHSAV LHNSTMYVFG GFNSLLLSDI LVFTSEQCDA HRSEAACLAA661GPGIRCVWNT GSSQCISWAL ATDEQEEKLK SECFSKRTLD HDRCDQHTDC YSCTANTNDC721HWCNDHCVPR NHSCSEGQIS IFRYENCPKD NPMYYCNKKT SCRSCALDQN CQWEPRNQEC781IALPENICGI GWHLVGNSCL KITTAKENYD NAKLFCRNHN ALLASLTTQK KVEFVLKQLR841IMQSSQSMSK LTLTPWVGLR KINVSYWCWE DMSPFTNSLL QWMPSEPSDA GFCGILSEPS901TRGLKAATCI NPLNGSVCER PANHSAKQCR TPCALRTACG DCTSGSSECM WCSNMKQCVD961SNAYVASFPF GQCMEWYTMS TCPPENCSGY CTCSHCLEQP GCGWCTDPSN TGKGKCIEGS1021YKGPVKMPSQ APTGNFYPQP LLNSSMCLED SRYNWSFIHC PACQCNGHSK CINQSICEKC1081ENLTTGKHCE TCISGFYGDP TNGGKCQPCK CNGHASLCNT NTGKCFCTTK GVKGDECQLC1141EVENRYQGNP LRGTCYYTLL IDYQFTFSLS QEDDRYYTAI NFVATPDEQN RDLDMFINAS1201KNFNLNITWA ASFSAGTQAG EEMPVVSKTN IKEYKDSFSN EKFDFRNHPN ITFFVYVSNF1261TWPIKIQVQT EQSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1272 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.