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ATRIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATRIP Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ATRIP antibodies →
$725.00each
Catalog Number: LS-G107683-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATRIP (NM_130384), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AGS1 | ATR-interacting protein | ATRIP | ATR interacting protein
Origin Species
Human
NCBI GenBank
NM_130384
SwissProt
Q8WXE1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGTSAPGSK RRSEPPAPRP GPPPGTGHPP SKRARGFSAA AAPDPDDPFG AHGDFTADDL
61EELDTLASQA LSQCPAAARD VSSDHKVHRL LDGMSKNPSG KNRETVPIKD NFELEVLQAQ
121YKELKEKMKV MEEEVLIKNG EIKILRDSLH QTESVLEEQR RSHFLLEQEK TQALSDKEKE
181FSKKLQSLQS ELQFKDAEMN ELRTKLQTSE RANKLAAPSV SHVSPRKNPS VVIKPEACSP
241QFGKTSFPTK ESFSANMSLP HPCQTESGYK PLVGREDSKP HSLRGDSIKQ EEAQKSFVDS
301WRQRSNTQGS ILINLLLKQP LIPGSSLSLC HLLSSSSESP AGTPLQPPGF GSTLAGMSGL
361RTTGSYDGSF SLSALREAQN LAFTGLNLVA RNECSRDGDP AEGGRRAFPL CQLPGAVHFL
421PLVQFFIGLH CQALQDLAAA KRSGAPGDSP THSSCVSSGV ETNPEDSVCI LEGFSVTALS
481ILQHLVCHSG AVVSLLLSGV GADSAAGEGN RSLVHRLSDG DMTSALRGVA DDQGQHPLLK
541MLLHLLAFSS AATGHLQASV LTQCLKVLVK LAENTSCDFL PRFQCVFQVL PKCLSPETPL
601PSVLLAVELL SLLADHDQLA PQLCSHSEGC LLLLLYMYIT SRPDRVALET QWLQLEQEVV
661WLLAKLGVQS PLPPVTGSNC QCNVEVVRAL TVMLHRQWLT VRRAGGPPRT DQQRRTVRCL
721RDTVLLLHGL SQKDKLFMMH CVEVLHQFDQ VMPGVSMLIR GLPDVTDCEE AALDDLCAAE
781TDVEDPEVEC G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

791 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.