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ATP8B4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATP8B4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ATP8B4 antibodies →
$725.00each
Catalog Number: LS-G109672-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATP8B4 (NM_024837), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATP8B4 | ATPIM | KIAA1939
Origin Species
Human
NCBI GenBank
AK025125

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
135.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFCSEKKLRE VERIVKANDR EYNEKFQYAD NRIHTSKYNI LTFLPINLFE QFQRVANAYF
61LCLLILQLIP EISSLTWFTT IVPLVLVITM TAVKDATDDY FRHKSDNQVN NRQSEVLINS
121KLQNEKWMNV KVGDIIKLEN NQFVAADLLL LSSSEPHGLC YVETAELDGE TNLKVRHALS
181VTSELGADIS RLAGFDGIVV CEVPNNKLDK FMGILSWKDS KHSLNNEKII LRGCILRNTS
241WCFGMVIFAG PDTKLMQNSG KTKFKRTSID RLMNTLVLWI FGFLICLGII LAIGNSIWES
301QTGDQFRTFL FWNEGEKSSV FSGFLTFWSY IIILNTVVPI SLYVSVEVIR LGHSYFINWD
361RKMYYSRKAI PAVARTTTLN EELGQIEYIF SDKTGTLTQN IMTFKRCSIN GRIYGEVHDD
421LDQKTEITQE KEPVDFSVKS QADREFQFFD HHLMESIKMG DPKVHEFLRL LALCHTVMSE
481ENSAGELIYQ VQSPDEGALV TAARNFGFIF KSRTPETITI EELGTLVTYQ LLAFLDFNNT
541RKRMSVIVRN PEGQIKLYSK GADTILFEKL HPSNEVLLSL TSDHLSEFAG EGLRTLAIAY
601RDLDDKYFKE WHKMLEDANA ATEERDERIA GLYEEIERDL MLLGATAVED KLQEGVIETV
661TSLSLANIKI WVLTGDKQET AINIGYACNM LTDDMNDVFV IAGNNAVEVR EELRKAKQNL
721FGQNRNFSNG HVVCEKKQQL ELDSIVEETI TGDYALIING HSLAHALESD VKNDLLELAC
781MCKTVICCRV TPLQKAQVVE LVKKYRNAVT LAIGDGANDV SMIKSAHIGV GISGQEGLQA
841VLASDYSFAQ FRYLQRLLLV HGRWSYFRMC KFLCYFFYKN FAFTLVHFWF GFFCGFSAQT
901VYDQWFITLF NIVYTSLPVL AMGIFDQDVS DQNSVDCPQL YKPGQLNLLF NKRKFFICVL
961HGIYTSLVLF FIPYGAFYNV AGEDGQHIAD YQSFAVTMAT SLVIVVSVQI ALDTSYWTFI
1021NHVFIWGSIA IYFSILFTMH SNGIFGIFPN QFPFVGNARH SLTQKCIWLV ILLTTVASVM
1081PVVAFRFLKV DLYPTLSDQI RRWQKAQKKA RPPSSRRPRT RRSSSRRSGY AFAHQEGYGE
1141LITSGKNMRA KNPPPTSGLE KTHYNSTSWI ENLCKKTTDT VSSFSQDKTV KL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1192 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.