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ATP8B3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATP8B3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107208-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATP8B3 (NM_138813), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATP8B3 | ATPIK | FOS37502_2 | ATP1K
Origin Species
Human
NCBI GenBank
AA827939
SwissProt
O60423

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
146.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGTGPAQTPR STRAGPEPSP APPGPGDTGD SDVTQEGSGP AGIRGGETVI RAGMGDSPGR
61GAPERRHKAQ PGRARKYEWR PEGPTSMGSL GQREDLQDED RNSAFTWKVQ ANNRAYNGQF
121KEKVILCWQR KKYKTNVIRT AKYNFYSFLP LNLYEQFHRV SNLFFLIIII LQSIPDISTL
181PWFSLSTPMV CLLFIRATRD LVDDMGRHKS DRAINNRPCQ ILMGKSFKQK KWQDLCVGDV
241VCLRKDNIVP ADMLLLASTE PSSLCYVETV DIDGETNLKF RQALMVTHKE LATIKKMASF
301QGTVTCEAPN SRMHHFVGCL EWNDKKYSLD IGNLLLRGCR IRNTDTCYGL VIYAGFDTKI
361MKNCGKIHLK RTKLDLLMNK LVVVIFISVV LVCLVLAFGF GFSVKEFKDH HYYLSGVHGS
421SVAAESFFVF WSFLILLSVT IPMSMFILSE FIYLGNSVFI DWDVQMYYKP QDVPAKARST
481SLNDHLGQVE YIFSDKTGTL TQNILTFNKC CISGRVYGPD SEATTRPKEN PYLWNKFADG
541KLLFHNAALL HLVRTNGDEA VREFWRLLAI CHTVMVRESP RERPDQLLYQ AASPDEGALV
601TAARNFGYVF LSRTQDTVTI MELGEERVYQ VLAIMDFNST RKRMSVLVRK PEGAICLYTK
661GADTVIFERL HRRGAMEFAT EEALAAFAQE TLRTLCLAYR EVAEDIYEDW QQRHQEASLL
721LQNRAQALQQ LLGATAIEDR LQDGVPETIK CLKKSNIKIW VLTGDKQETA VNIGFACELL
781SENMLILEEK EISRILETYW ENSNNLLTRE SLSQVKLALV INGDFLDKLL VSLRKEPRAL
841AQNVNMDEAW QELGQSRRDF LYARRLSLLC RRFGLPLAAP PAQDSRARRS SEVLQERAFV
901DLASKCQAVI CCRVTPKQKA LIVALVKKYH QVVTLAIGDG ANDINMIKTA DVGVGLAGQE
961GMQAVQNSDF VLGQFCFLQR LLLVHGRWSY VRICKFLRYF FYKSMASMMV QVWFACYNGF
1021TGQPLYEGWF LALFNLLYST LPVLYIGLFE QDVSAEQSLE KPELYVVGQK DELFNYWVFV
1081QAIAHGVTTS LVNFFMTLWI SRDTAGPASF SDHQSFAVVV ALSCLLSITM EVILIIKYWT
1141ALCVATILLS LGFYAIMTTT TQSFWLFRVS PTTFPFLYAD LSVMSSPSIL LVVLLSVSIN
1201TFPVLALRVI FPALKELRAK EEKVEEGPSE EIFTMEPLPH VHRESRARRS SYAFSHREGY
1261ANLITQGTIL RRGPGVSSDI ASESLDPSDE EAASSPKESQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1300 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.