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ATP13A4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATP13A4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ATP13A4 antibodies →
$725.00each
Catalog Number: LS-G108917-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATP13A4 (NM_032279), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cation-transporting P5-ATPase | p5-ATPase isoform 4 | ATP13A4 | ATPase type 13A4 | p5-ATPase
Origin Species
Human
NCBI GenBank
NM_032279
SwissProt
Q4VNC1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGHFEKGQHA LLNEGEENEM EIFGYRTQGC RKSLCLAGSI FSFGILPLVF YWRPAWHVWA
61HCVPCSLQEA DTVLLRTTDE FQIYSWKKVI WIYLSALNSA FGLTPDHPLM TDEEYIINRA
121IRKPDLKVRC IKVQKIRYVW NYLEGQFQKI GSLEDWLSSA KIHQKFGSGL TREEQEIRRL
181ICGPNTIDVE VTPIWKLLIK EVLNPFYIFQ LFSVCLWFSE DYKEYAFAII IMSIISISLT
241VYDLREQSVK LHHLVESHNS ITVSVCGRKA GVQELESRVL VPGDLLILTG NKVLMPCDAV
301LIEGSCVVDE GMLTGESIPV TKTPLPKMDS SVPWKTQSEA DYKRHVLFCG TEVIQAKAAC
361SGTVRAVVLQ TGFNTAKGDL VRSILYPKPV NFQLYRDAIR FLLCLVGTAT IGMIYTLCVY
421VLSGEPPEEV VRKALDVITI AVPPALPAAL TTGIIYAQRR LKKRGIFCIS PQRINVCGQL
481NLVCFDKTGT LTRDGLDLWG VVSCDRNGFQ EVHSFASGQA LPWGPLCAAM ASCHSLILLD
541GTIQGDPLDL KMFEATTWEM AFSGDDFHIK GVPAHAMVVK PCRTASQVPV EGIAILHQFP
601FSSALQRMTV IVQEMGGDRL AFMKGAPERV ASFCQPETVP TSFVSELQIY TTQGFRVIAL
661AYKKLENDHH ATTLTRETVE SDLIFLGLLI LENRLKEETK PVLEELISAR IRTVMITGDN
721LQTAITVARK SGMVSESQKV ILIEANETTG SSSASISWTL VEEKKHIMYG NQDNYINIRD
781EVSDKGREGS YHFALTGKSF HVISQHFSSL LPKILINGTI FARMSPGQKS SLVEEFQKLD
841YFVGMCGDGA NDCGALKMAH VGISLSEQEA SVASPFTSKT PNIECVPHLI KEGRAALVTS
901FCMFKYMALY SMIQYVGVLL LYWETNSLSN YQFLFQDLAI TTLIGVTMNL NGAYPKLVPF
961RPAGRLISPP LLLSVIFNIL LSLAMHIAGF ILVQRQPWYS VEIHSACTVQ NESISELTMS
1021PTAPEKMESN STFTSFENTT VWFLGTINCI TVALVFSKGK PFRQPTYTNY IFVLVLIIQL
1081GVCLFILFAD IPELYRRLDL LCTPVLWRAS IVIMLSLNFI VSLVAEEAVI ENRALWMMIK
1141RCFGYQSKSQ YRIWQRDLAN DPSWPPLNQT SHSDMPECGR GVSYSNPVFE SNEEQL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1196 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.