Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ATG18 / WIPI1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101519-100
Catalog Number: LS-G101519
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WIPI1 (NM_017983), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATG18 | Atg18 protein homolog | ATG18A | WIPI49 | WIPI-1 | WIPI1 | WIPI 49 kDa | WIPI-1 alpha
- Origin Species
- Human
- SwissProt
- Q5MNZ9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAEAADAPP GGVESALSCF SFNQDCTSLA IGTKAGYKLF SLSSVEQLDQ VHGSNEIPDV61YIVERLFSSS LVVVVSHTKP RQMNVYHFKK GTEICNYSYS SNILSIRLNR QRLLVCLEES121IYIHNIKDMK LLKTLLDIPA NPTGLCALSI NHSNSYLAYP GSLTSGEIVL YDGNSLKTVC181TIAAHEGTLA AITFNASGSK LASASEKGTV IRVFSVPDGQ KLYEFRRGMK RYVTISSLVF241SMDSQFLCAS SNTETVHIFK LEQVTNSRPE EPSTWSGYMG KMFMAATNYL PTQVSDMMHQ301DRAFATARLN FSGQRNICTL STIQKLPRLL VASSSGHLYM YNLDPQDGGE CVLIKTHSLL361GSGTTEENKE NDLRPSLPQS YAATVARPSA SSASTVPGYS EDGGALRGEV IPEHEFATGP421VCLDDENEFP PIILCRGNQK GKTKQSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
446 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.