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ATG16L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATG16L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ATG16L2 antibodies →
$725.00each
Catalog Number: LS-G108270-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATG16L2 (NM_033388), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATG16L2 | ATG16B | Autophagy-related protein 16-2 | WDR80 | APG16-like 2
Origin Species
Human
NCBI GenBank
AK024423
SwissProt
Q8NAA4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGPGVPGAP AARWKRHIVR QLRLRDRTQK ALFLELVPAY NHLLEKAELL DKFSKKLQPE
61PNSVTPTTHQ GPWEESELDS DQVPSLVALR VKWQEEEEGL RLVCGEMAYQ VVEKGAALGT
121LESELQQRQS RLAALEARVA QLREARAQQA QQVEEWRAQN AVQRAAYEAL RAHVGLREAA
181LRRLQEEARD LLERLVQRKA RAAAERNLRN ERRERAKQAR VSQELKKAAK RTVSISEGPD
241TLGDGMRERR ETLALAPEPE PLEKEACEKW KRPFRSASAT SLTLSHCVDV VKGLLDFKKR
301RGHSIGGAPE QRYQIIPVCV AARLPTRAQD VLDAHLSEVN AVRFGPNSSL LATGGADRLI
361HLWNVVGSRL EANQTLEGAG GSITSVDFDP SGYQVLAATY NQAAQLWKVG EAQSKETLSG
421HKDKVTAAKF KLTRHQAVTG SRDRTVKEWD LGRAYCSRTI NVLSYCNDVV CGDHIIISGH
481NDQKIRFWDS RGPHCTQVIP VQGRVTSLSL SHDQLHLLSC SRDNTLKVID LRVSNIRQVF
541RADGFKCGSD WTKAVFSPDR SYALAGSCDG ALYIWDVDTG KLESRLQGPH CAAVNAVAWC
601YSGSHMVSVD QGRKVVLWQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

619 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.