Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ATG16L1 / ATG16L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G131014-100
Catalog Number: LS-G131014
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATG16L1 (NM_001190266), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APG16-like 1 | APG16L | APG16L beta | ATG16L1 | ATG16L | Autophagy-related protein 16-1 | IBD10 | WD repeat domain 30 | ATG16A | WDR30
- Origin Species
- Human
- NCBI GenBank
- NM_017974
- SwissProt
- Q676U5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQLRIKHQE ELTELHKKRG ELAQLVIDLN NQMQRKDREM QMNEAKIAEC LQTISDLETE61CLDLRTKLCD LERANQTLKD EYDALQITFT ALEGKLRKTT EENQELVTRW MAEKAQEANR121LNAENEKDSR RRQARLQKEL AEAAKEPLPV EQDDDIEVIV DETSDHTEET SPVRAISRAA181TKRLSQPAGG LLDSITNIFG RRSVSSFPVP QDNVDTHPGS GKEVRVPATA LCVFDAHDGE241VNAVQFSPGS RLLATGGMDR RVKLWEVFGE KCEFKGSLSG SNAGITSIEF DSAGSYLLAA301SNDFASRIWT VDDYRLRHTL TGHSGKVLSA KFLLDNARIV SGSHDRTLKL WDLRSKVCIK361TVFAGSSCND IVCTEQCVMS GHFDKKIRFW DIRSESIVRE MELLGKITAL DLNPERTELL421SCSRDDLLKV IDLRTNAIKQ TFSAPGFKCG SDWTRVVFSP DGSYVAAGSA EGSLYIWSVL481TGKVEKVLSK QHSSSINAVA WSPSGSHVVS VDKGCKAVLW AQYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
523 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.