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ATE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ATE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ATE1 antibodies →
$504.00each
Catalog Number: LS-G114904-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ATE1 (NM_007041), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATE1 | Arginyltransferase 1 | R-transferase 1
Origin Species
Human
NCBI GenBank
AF079098
SwissProt
O95260

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFWAGGSPS VVDYFPSEDF YRCGYCKNES GSRSNGMWAH SMTVQDYQDL IDRGWRRSGK
61YVYKPVMNQT CCPQYTIRCR PLQFQPSKSH KKVLKKMLKF LAKGEVPKGS CEDEPMDSTM
121DDAVAGDFAL INKLDIQCDL KTLSDDIKES LESEGKNSKK EEPQELLQSQ DFVGEKLGSG
181EPSHSVKVHT VPKPGKGADL SKPPCRKAKE IRKERKRLKL MQQNPAGELE GFQAQGHPPS
241LFPPKAKSNQ PKSLEDLIFE SLPENASHKL EVRLVPVSFE DPEFKSSFSQ SFSLYVKYQV
301AIHQDPPDEC GKTEFTRFLC SSPLEAETPP NGPDCGYGSF HQQYWLDGKI IAVGVIDILP
361NCVSSVYLYY DPDYSFLSLG VYSALREIAF TRQLHEKTSQ LSYYYMGFYI HSCPKMKYKG
421QYRPSDLLCP ETYVWVPIEQ CLPSLENSKY CRFNQDPEAV DEDRSTEPDR LQVFHKRAIM
481PYGVYKKQQK DPSEEAAVLQ YASLVGQKCS ERMLLFRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.