Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ATAC1 / ZZZ3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113153-100
Catalog Number: LS-G113153
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZZZ3 (NM_015534), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZZZ3 | ATAC component 1 homolog | ATAC1 | DKFZP564I052
- Origin Species
- Human
- NCBI GenBank
- AL080063
- SwissProt
- Q8IYH5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAASRSTRVT RSTVGLNGLD ESFCGRTLRN RSIAHPEEIS SNSQVRSRSP KKRPEPVPIQ61KGNNNGRTTD LKQQSTRESW VSPRKRGLSS SEKDNIERQA IENCERRQTE PVSPVLKRIK121RCLRSEAPNS SEEDSPIKSD KESVEQRSTV VDNDADFQGT KRACRCLILD DCEKREIKKV181NVSEEGPLNS AVVEEITGYL AVNGVDDSDS AVINCDDCQP DGNTKQNSIG SYVLQEKSVA241ENGDTDTQTS MFLDSRKEDS YIDHKVPCTD SQVQVKLEDH KIVTACLPVE HVNQLTTEPA301TGPFSETQSS LRDSEEEVDV VGDSSASKEQ CKENTNNELD TSLESMPASG EPEPSPVLDC361VSAQMMSLSE PQEHRYTLRT SPRRAAPTRG SPTKNSSPYR ENGQFEENNL SPNETNATVS421DNVSQSPTNP GEISQNEKGI CCDSQNNGSE GVSKPPSEAR LNIGHLPSAK ESASQHITEE481EDDDPDVYYF ESDHVALKHN KDYQRLLQTI AVLEAQRSQA VQDLESLGRH QREALKNPIG541FVEKLQKKAD IGLPYPQRVV QLPEIVWDQY THSLGNFERE FKNRKRHTRR VKLVFDKVGL601PARPKSPLDP KKDGESLSYS MLPLSDGPEG SSSRPQMIRG RLCDDTKPET FNQLWTVEEQ661KKLEQLLIKY PPEEVESRRW QKIADELGNR TAKQVASRVQ KYFIKLTKAG IPVPGRTPNL721YIYSKKSSTS RRQHPLNKHL FKPSTFMTSH EPPVYMDEDD DRSCFHSHMN TAVEDASDDE781SIPIMYRNLP EYKELLQFKK LKKQKLQQMQ AESGFVQHVG FKCDNCGIEP IQGVRWHCQD841CPPEMSLDFC DSCSDCLHET DIHKEDHQLE PIYRSETFLD RDYCVSQGTS YNYLDPNYFP901ANRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
903 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.