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ASXL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASXL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ASXL2 antibodies →
$725.00each
Catalog Number: LS-G111851-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASXL2 (NM_018263), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASXH2 | Polycomb group protein ASXH2 | KIAA1685 | ASXL2
Origin Species
Human
NCBI GenBank
NM_018263
SwissProt
Q76L83

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
153.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MREKGRRKKG RTWAEAAKTV LEKYPNTPMS HKEILQVIQR EGLKEIRSGT SPLACLNAML
61HTNSRGEEGI FYKVPGRMGV YTLKKDVPDG VKELSEGSEE SSDGQSDSQS SENSSSSSDG
121GSNKEGKKSR WKRKVSSSSP QSGCPSPTIP AGKVISPSQK HSKKALKQAL KQQQQKKQQQ
181QCRPSISISS NQHLSLKTVK AASDSVPAKP ATWEGKQSDG QTGSPQNSNS SFSSSVKVEN
241TLLGLGKKSF QRSERLHTRQ MKRTKCADID VETPDSILVN TNLRALINKH TFSVLPGDCQ
301QRLLLLLPEV DRQVGPDGLM KLNGSALNNE FFTSAAQGWK ERLSEGEFTP EMQVRIRQEI
361EKEKKVEPWK EQFFESYYGQ SSGLSLEDSK KLTASPSDPK VKKTPAEQPK SMPVSEASLI
421RIVPVVSQSE CKEEALQMSS PGRKEECESQ GEVQPNFSTS SEPLLSSALN THELSSILPI
481KCPKDEDLLE QKPVTSAEQE SEKNHLTTAS NYNKSESQES LVTSPSKPKS PGVEKPIVKP
541TAGAGPQETN MKEPLATLVD QSPESLKRKS SLTQEEAPVS WEKRPRVTEN RQHQQPFQVS
601PQPFLNRGDR IQVRKVPPLK IPVSRISPMP FHPSQVSPRA RFPVSITSPN RTGARTLADI
661KAKAQLVKAQ RAAAAAAAAA AAAASVGGTI PGPGPGGGQG PGEGGEGQTA RGGSPGSDRV
721SETGKGPTLE LAGTGSRGGT RELLPCGPET QPQSETKTTP SQAQPHSVSG AQLQQTPPVP
781PTPAVSGACT SVPSPAHIEK LDNEKLNPTR ATATVASVSH PQGPSSCRQE KAPSPTGPAL
841ISGASPVHCA ADGTVELKAG PSKNIPNPSA SSKTDASVPV AVTPSPLTSL LTTATLEKLP
901VPQVSATTAP AGSAPPSSTL PAASSLKTPG TSLNMNGPTL RPTSSIPANN PLVTQLLQGK
961DVPMEQILPK PLTKVEMKTV PLTAKEERGM GALIATNTTE NSTREEVNER QSHPATQQQL
1021GKTLQSKQLP QVPRPLQLFS AKELRDSSID THQYHEGLSK ATQDQILQTL IQRVRRQNLL
1081SVVPPSQFNF AHSGFQLEDI STSQRFMLGF AGRRTSKPAM AGHYLLNIST YGRGSESFRR
1141THSVNPEDRF CLSSPTEALK MGYTDCKNAT GESSSSKEDD TDEESTGDEQ ESVTVKEEPQ
1201VSQSAGKGDT SSGPHSRETL STSDCLASKN VKAEIPLNEQ TTLSKENYLF TRGQTFDEKT
1261LARDLIQAAQ KQMAHAVRGK AIRSSPELFS STVLPLPADS PTHQPLLLPP LQTPKLYGSP
1321TQIGPSYRGM INVSTSSDMD HNSAVPGSQV SSNVGDVMSF SVTVTTIPAS QAMNPSSHGQ
1381TIPVQAFSEE NSIEGTPSKC YCRLKAMIMC KGCGAFCHDD CIGPSKLCVS CLVVR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1435 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.