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ASTE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASTE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114155-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASTE1 (NM_014065), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HT001 | Protein asteroid homolog 1 | ASTE1
Origin Species
Human
NCBI GenBank
NM_014065
SwissProt
Q2TB18

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGIRGLMSFV EDHSNEFFTD LKLRDTKIVI DGYALFHRLC FSSNLDLRYG GDYDSFADVV
61QKFFESLFAC NICPYVVLDG GCDISDKKLT TLKDRAREKI QMAHSLSVGG SGYVCPLLIR
121EVFIQVLIKL RVCFVQCFSE ADRDIMTLAN HWNCPVLSSD SDFCIFDLKT GFCPLNSFQW
181RNMNTIKGTQ NYIPAKCFSL DAFCHHFSNM NKALLPLFAV LCGNDHVNLP IMETFLSKAR
241LPLGATSSKG RRHHRILGLL NWLSHFANPT EALDNVLKYL PKKDRENVKE LLCCSMEEYQ
301QSQVKLQDFF QCGTYVCPDA LNLGLPEWVL VALAKGQLSP FISDALVLRR TILPTQVENM
361QQPNAHRISQ PIRQIIYGLL LNASPHLDKT SWNALPPQPL AFSEVERINK NIRTSIIDAV
421ELAKDHSDLS RLTELSLRRR QMLLLETLKV KQTILEPIPT SLKLPIAVSC YWLQHTETKA
481KLHHLQSLLL TMLVGPLIAI INSPGKEELQ EDGAKMLYAE FQRVKAQTRL GTRLDLDTAH
541IFCQWQSCLQ MGMYLNQLLS TPLPEPDLTR LYSGSLVHGL CQQLLASTSV ESLLSICPEA
601KQLYEYLFNA TRSYAPAEIF LPKGRSNSKK KRQKKQNTSC SKNRGRTTAH TKCWYEGNNR
661FGLLMVENLE EHSEASNIE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

679 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.