Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ASPIC1 / CRTAC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112020-100
Catalog Number: LS-G112020
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CRTAC1 (NM_018058), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASPIC1 | ASPIC | CRTAC1 | CEP-68 | Cartilage acidic protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_018058
- SwissProt
- Q9NQ79
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPSADPGMS RMLPFLLLLW FLPITEGSQR AEPMFTAVTN SVLPPDYDSN PTQLNYGVAV61TDVDHDGDFE IVVAGYNGPN LVLKYDRAQK RLVNIAVDER SSPYYALRDR QGNAIGVTAC121DIDGDGREEI YFLNTNNAFS GVATYTDKLF KFRNNRWEDI LSDEVNVARG VASLFAGRSV181ACVDRKGSGR YSIYIANYAY GNVGPDALIE MDPEASDLSR GILALRDVAA EAGVSKYTGG241RGVSVGPILS SSASDIFCDN ENGPNFLFHN RGDGTFVDAA ASAGVDDPHQ HGRGVALADF301NRDGKVDIVY GNWNGPHRLY LQMSTHGKVR FRDIASPKFS MPSPVRTVIT ADFDNDQELE361IFFNNIAYRS SSANRLFRVI RREHGDPLIE ELNPGDALEP EGRGTGGVVT DFDGDGMLDL421ILSHGESMAQ PLSVFRGNQG FNNNWLRVVP RTRFGAFARG AKVVLYTKKS GAHLRIIDGG481SGYLCEMEPV AHFGLGKDEA SSVEVTWPDG KMVSRNVASG EMNSVLEILY PRDEDTLQDP541APLECGQGFS QQENGHCMDT NECIQFPFVC PRDKPVCVNT YGSYRCRTNK KCSRGYEPNE601DGTACVGTLG QSPGPRPTTP TAAAATAAAA AAAGAATAAP VLVDGDLNLG SVVKESCEPS661CTRTRPLEQKLISEEDLAANDILDYKDDDDKV
661 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.