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ASPG Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASPG Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119337-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASPG (NM_001080464), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
60 kDa lysophospholipase | ASPG | GPA/WT | C14orf76
Origin Species
Human
NCBI GenBank
NM_001080464

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH ARARGLSEDT
61LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI KRHYEQYHGF VVIHGTDTMA
121FAASMLSFML ENLQKTVILT GAQVPIHALW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL
181FRGNRATKVD ARRFAAFCSP NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL
241LRLYPGIPAA LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL
301QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE LLTKDLRGEM
361TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL VPSLACAAAH AGDVEALQAL
421VELGSDLGLV DFNGQTPLHA AARGGHTEAV TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP
481GVIGLLREAG ASLSTQELEE AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV
541AEAAGNLAVV AFLQSLEGAV GAQAPCPEPS CFQEVLPGV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

579 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.