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ASNSD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASNSD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111470-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASNSD1 (NM_019048), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NBLA00058 | ASNSD1 | NS3TP1
Origin Species
Human
NCBI GenBank
NM_019048
SwissProt
Q9NWL6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCGICCSVNF SAEHFSQDLK EDLLYNLKQR GPNSSKQLLK SDVNYQCLFS AHVLHLRGVL
61TTQPVEDERG NVFLWNGEIF SGIKVEAEEN DTQILFNYLS SCKNESEILS LFSEVQGPWS
121FIYYQASSHY LWFGRDFFGR RSLLWHFSNL GKSFCLSSVG TQTSGLANQW QEVPASGLFR
181IDLKSTVISR CIILQLYPWK YISRENIIEE NVNSLSQISA DLPAFVSVVA NEAKLYLEKP
241VVPLNMMLPQ AALETHCSNI SNVPPTREIL QVFLTDVHMK EVIQQFIDVL SVAVKKRVLC
301LPRDENLTAN EVLKTCDRKA NVAILFSGGI DSMVIATLAD RHIPLDEPID LLNVAFIAEE
361KTMPTTFNRE GNKQKNKCEI PSEEFSKDVA AAAADSPNKH VSVPDRITGR AGLKELQAVS
421PSRIWNFVEI NVSMEELQKL RRTRICHLIR PLDTVLDDSI GCAVWFASRG IGWLVAQEGV
481KSYQSNAKVV LTGIGADEQL AGYSRHRVRF QSHGLEGLNK EIMMELGRIS SRNLGRDDRV
541IGDHGKEARF PFLDENVVSF LNSLPIWEKA NLTLPRGIGE KLLLRLAAVE LGLTASALLP
601KRAMQFGSRI AKMEKINEKA SDKCGRLQIM SLENLSIEKE TKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

643 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.