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ASMTL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASMTL Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ASMTL antibodies →
$504.00each
Catalog Number: LS-G130069-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASMTL (NM_001173474), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASTML | ASMTLY | ASMTL | ASMTLX
Origin Species
Human
NCBI GenBank
NM_004192
SwissProt
O95671

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLCPVIGKL LHKRVVLASA SPRRQEILSN AGLRFEVVPS KFKEKLDKAS FATPYGYAME
61TAKQKALEVA NRLYQTVGGL ILEKPVDKQD AYRMLSRLSG REHSVFTGVA IVHCSSKDHQ
121LDTRVSEFYE ETKVKFSELS EELLWEYVHS GEPMDKAGGY GIQALGGMLV ESVHGDFLNV
181VGFPLNHFCK QLVKLYYPPR PEDLRRSVKH DSIPAADTFE DLSDVEGGGS EPTQRDAGSR
241DEKAEAGEAG QATAEAECHR TRETLPPFPT RLLELIEGFM LSKGLLTACK LKVFDLLKDE
301APQKAADIAS KVDASACGME RLLDICAAMG LLEKTEQGYS NTETANVYLA SDGEYSLHGF
361IMHNNDLTWN LFTYLEFAIR EGTNQHHRAL GKKAEDLFQD AYYQSPETRL RFMRAMHGMT
421KLTACQVATA FNLSRFSSAC DVGGCTGALA RELAREYPRM QVTVFDLPDI IELAAHFQPP
481GPQAVQIHFA AGDFFRDPLP SAELYVLCRI LHDWPDDKVH KLLSRVAESC KPGAGLLLVE
541TLLDEEKRVA QRALMQSLNM LVQTEGKERS LGEYQCLLEL HGFHQVQVVH LGGVLDAILA
601TKVAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

605 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.