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ASB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101385-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASB3 (NM_016115), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASB-3 | ASB3
Origin Species
Human
NCBI GenBank
NM_016115
SwissProt
Q9Y575

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDFTEAYADT CSTVGLAARE GNVKVLRKLL KKGRSVDVAD NRGWMPIHEA AYHNSVECLQ
61MLINADSSEN YIKMKTFEGF CALHLAASQG HWKIVQILLE AGADPNATTL EETTPLFLAV
121ENGQIDVLRL LLQHGANVNG SHSMCGWNSL HQASFQENAE IIKLLLRKGA NKECQDDFGI
181TPLFVAAQYG KLESLSILIS SGANVNCQAL DKATPLFIAA QEGHTKCVEL LLSSGADPDL
241YCNEDSWQLP IHAAAQMGHT KILDLLIPLT NRACDTGLNK VSPVYSAVFG GHEDCLEILL
301RNGYSPDAQA CLVFGFSSPV CMAFQKDCEF FGIVNILLKY GAQINELHLA YCLKYEKFSI
361FRYFLRKGCS LGPWNHIYEF VNHAIKAQAK YKEWLPHLLV AGFDPLILLC NSWIDSVSID
421TLIFTLEFTN WKTLAPAVER MLSARASNAW ILQQHIATVP SLTHLCRLEI RSSLKSERLR
481SDSYISQLPL PRSLHNYLLY EDVLRMYEVP ELAAIQDG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.