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ASB15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASB15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ASB15 antibodies →
$725.00each
Catalog Number: LS-G107679-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASB15 (NM_080928), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASB15 | ASB-15
Origin Species
Human
NCBI GenBank
NM_080928
SwissProt
Q8WXK1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDTNDDPDED HLTSYDIQLS IQESIEASKT ALCPERFVPL SAQNRKLVEA IKQGHIPELQ
61EYVKYKYAMD EADEKGWFPL HEAVVQPIQQ ILEIVLDASY KTLWEFKTCD GETPLTLAVK
121AGLVENVRTL LEKGVWPNTK NDKGETPLLI AVKKGSYDMV STLIKHNTSL DQPCVKRWSA
181MHEAAKQGRK DIVALLLKHG GNVHLRDGFG VTPLGVAAEY GHCDVLEHLI HKGGDVLALA
241DDGASVLFEA AGGGNPDCIS LLLEYGGSGN VPNRAGHLPI HRAAYEGHYL ALKYLIPVTS
301KNAIRKSGLT PIHSAADGQN AQCLELLIEN GFDVNTLLAD HISQSYDDER KTALYFGVSN
361NDVHCTEVLL AAGADPNLDP LNCLLVAVRA NNYEIVRLLL SHGANVNCYF MHVNDTRFPS
421VIQYALNDEV MLRLLLNNGY QVEMCFDCMH GDIFGNSFVW SEIQEEVLPG WTSCVIKDNP
481FCEFITVPWM KHLVGRVTRV LIDYMDYVPL CAKLKSALEV QREWPEIRQI LENPCSLKHL
541CRLKIRRLMG LQKLCQPASV EKLPLPPAIQ RYILFKEYDL YGQELKLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

588 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.