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ASAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ASAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112289-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASAP3 (NM_017707), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACAP4 | ARF6 GTPase-activating protein | ASAP3 | CENTB6 | DDEFL1 | Centaurin | beta 6 | UPLC1
Origin Species
Human
NCBI GenBank
NM_017707
SwissProt
Q8TDY4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPEQFSVAEF LAVTAEDLSS PAGAAAFAAK MPRYRGAALA REEILEGDQA ILQRIKKAVR
61AIHSSGLGHV ENEEQYREAV ESLGNSHLSQ NSHELSTGFL NLAVFTREVA ALFKNLIQNL
121NNIVSFPLDS LMKGQLRDGR QDSKKQLEKA WKDYEAKMAK LEKERDRARV TGGIPGEVAQ
181DMQRERRIFQ LHMCEYLLKA GESQMKQGPD FLQSLIKFFH AQHNFFQDGW KAAQSLFPFI
241EKLAASVHAL HQAQEDELQK LTQLRDSLRG TLQLESREEH LSRKNSGCGY SIHQHQGNKQ
301FGTEKVGFLY KKSDGIRRAW QKRKCGVKYG CLTISHSTIN RPPVKLTLLT CQVRPNPEEK
361KCFDLVTHNR TYHFQAEDEH ECEAWVSVLQ NSKDEALSSA FLGEPSAGPG SWGSAGHDGE
421PHDLTKLLIA EVKSRPGNSQ CCDCGAADPT WLSTNLGVLT CIQCSGVHRE LGVRFSRMQS
481LTLDLLGPSE LLLALNMGNT SFNEVMEAQL PSHGGPKPSA ESDMGTRRDY IMAKYVEHRF
541ARRCTPEPQR LWTAICNRDL LSVLEAFANG QDFGQPLPGP DAQAPEELVL HLAVKVANQA
601SLPLVDFIIQ NGGHLDAKAA DGNTALHYAA LYNQPDCLKL LLKGRALVGT VNEAGETALD
661IARKKHHKEC EELLEQAQAG TFAFPLHVDY SWVISTEPGS DSEEDEEEKR CLLKLPAQAH
721WASGRLDISN KTYETVASLG AATPQGESED CPPPLPVKNS SRTLVQGCAR HASGDRSEVS
781SLSSEAPETP ESLGSPASSS SLMSPLEPGD PSQAPPNSEE GLREPPGTSR PSLTSGTTPS
841EMYLPVRFSS ESTRSYRRGA RSPEDGPSAR QPLPRRNVPV GITEGDGSRT GSLPASSVQL
901LQD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

903 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.