Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ASAH1 / Acid Ceramidase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100258-100
Catalog Number: LS-G100258
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASAH1 (NM_004315), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACDase | Acid CDase | Acid ceramidase | ASAH | ASAH1 | Acylsphingosine deacylase | PHP32 | SMAPME | PHP | Putative 32 kDa heart protein
- Origin Species
- Human
- NCBI GenBank
- NM_004315
- SwissProt
- Q13510
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNCCIGLGEK ARGSHRASYP SLSALFTEAS ILGFGSFAVK AQWTEDCRKS TYPPSGPTYR61GAVPWYTINL DLPPYKRWHE LMLDKAPMLK VIVNSLKNMI NTFVPSGKVM QVVDEKLPGL121LGNFPGPFEE EMKGIAAVTD IPLGEIISFN IFYELFTICT SIVAEDKKGH LIHGRNMDFG181VFLGWNINND TWVITEQLKP LTVNLDFQRN NKTVFKASSF AGYVGMLTGF KPGLFSLTLN241ERFSINGGYL GILEWILGKK DAMWIGFLTR TVLENSTSYE EAKNLLTKTK ILAPAYFILG301GNQSGEGCVI TRDRKESLDV YELDAKQGRW YVVQTNYDRW KHPFFLDDRR TPAKMCLNRT361SQENISFETM YDVLSTKPVL NKLTVYTTLI DVTKGQFETY LRDCPDPCIG WTRTRPLEQKLISEEDLAANDILDYKDDDDKV
411 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.