Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARSH / Arylsulfatase H Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121807-100
Catalog Number: LS-G121807
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARSH (NM_001011719), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Arylsulfatase H | ASH | ARSH | Arylsulfatase family | member H | Sulfatase
- Origin Species
- Human
- SwissProt
- Q5FYA8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTRNARPNIV LLMADDLGVG DLCCYGNNSV STPNIDRLAS EGVRLTQHLA AASMCTPSRA61AFLTGRYPIR SGMVSAYNLN RAFTWLGGSG GLPTNETTFA KLLQHRGYRT GLIGKWHLGL121SCASRNDHCY HPLNHGFHYF YGVPFGLLSD CQASKTPELH RWLRIKLWIS TVALALVPFL181LLIPKFARWF SVPWKVIFVF ALLAFLFFTS WYSSYGFTRR WNCILMRNHE IIQQPMKEEK241VASLMLKEAL AFIERYKREP FLLFFSFLHV HTPLISKKKF VGRSKYGRYG DNVEEMDWMV301GKILDALDQE RLANHTLVYF TSDNGGHLEP LDGAVQLGGW NGIYKGGKGM GGWEGGIRVP361GIFRWPSVLE AGRVINEPTS LMDIYPTLSY IGGGILSQDR VIDGQNLMPL LEGRASHSDH421EFLFHYCGVY LHTVRWHQKD CATVWKAHYV TPKFYPEGTG ACYGSGICSC SGDVTYHDPP481LLFDISRDPS EALPLNPDNE PLFDSVIKKM EAAIREHRRT LTPVPQQFSV FNTIWKPWLQ541PCCGTFPFCG CDKEDDILPM APTRTRPLEQKLISEEDLAANDILDYKDDDDKV
562 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.