Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARRB1 / Beta Arrestin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111229-100
Catalog Number: LS-G111229
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human beta Arrestin 1(ARRB1) (NM_020251), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARRB1 | Arrestin 2 | Arrestin beta-1 | Arb1 | ARR1 | Beta-arrestin-1 | Arrestin | beta 1
- Origin Species
- Human
- NCBI GenBank
- NM_004041
- SwissProt
- P49407
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDKGTRVFK KASPNGKLTV YLGKRDFVDH IDLVDPVDGV VLVDPEYLKE RRVYVTLTCA61FRYGREDLDV LGLTFRKDLF VANVQSFPPA PEDKKPLTRL QERLIKKLGE HAYPFTFEIP121PNLPCSVTLQ PGPEDTGKAC GVDYEVKAFC AENLEEKIHK RNSVRLVIRK VQYAPERPGP181QPTAETTRQF LMSDKPLHLE ASLDKEIYYH GEPISVNVHV TNNTNKTVKK IKISVRQYAD241ICLFNTAQYK CPVAMEEADD TVAPSSTFCK VYTLTPFLAN NREKRGLALD GKLKHEDTNL301ASSTLLREGA NREILGIIVS YKVKVKLVVS RGGDVAVELP FTLMHPKPKE EPPHREVPEN361ETPVDTNLIE LDTNDDDIVF EDFARQRLKG MKDDKEEEED GTGSPQLNNRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
410 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.