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ARMC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARMC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARMC5 antibodies →
$725.00each
Catalog Number: LS-G109783-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARMC5 (NM_024742), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Armadillo repeat containing 5 | ARMC5
Origin Species
Human
NCBI GenBank
NM_001105247
SwissProt
Q96C12

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAKPTLTD SLSFCLAQLA AAAGEALGGE KDPATNETPL SRALLALRTR HIKAAGGIER
61FRARGGLRPL LALLRRAAAA GSAPSQAGPG SAPSSAASGA SSPAPASGPA PSAVSSSSPT
121PPVRLRKTLD LALSILADCC TEGACRTEVR RLGGILPLVT ILQCMKTDSI QNRTARALGN
181LAMEPESCGD IHCAGAVPLL VESLTACQDS QCLQSVVRAL RNLADSPQHR LALAQQGAVR
241PLAELLATAP DAALTLALVR ALLELSRGCS RACAEQLSLG GGLGPLVSLA SHPKRAVREG
301TILILANLCA QGLIRPALGN AGGVEVLVDE LRQRRDPNGA SPTSQQPLVR AVCLLCREAI
361NRARLRDAGG LDLLMGLLRD PRASAWHPRI VAALVGFLYD TGALGRLQAL GLVPLLAGQL
421CGEAGEEEEE GREAASWDFP EERTPERAQG GSFRSLRSWL ISEGYATGPD DISPDWSPEQ
481CPPEPMEPAS PAPTPTSLRA PRTQRTPGRS PAAAIEEPWG REGPALLLLS RFSQAPDPSG
541ALVTGPALYG LLTYVTGAPG PPSPRALRIL SRLTCNPACL EAFVRSYGAA LLRAWLVLGV
601APDDWPAPRA RPTLHSRHRE LGEFPYPPVS LPPCESPSSP MASMGPEPHL PTHLSSPARP
661PDNLSPEWGG EQGVPVPPWA HRQSSAVSSA LALGPRYPNS RCSPAPRIWA GLCFFPGSAI
721GSALS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

725 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.