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ARMC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARMC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105408-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARMC3 (NM_173081), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARMC3 | Beta-catenin-like protein | KU-CT-1 | RP11-300B11.1 | Armadillo repeat containing 3 | Cancer/testis antigen 81 | CT81
Origin Species
Human
SwissProt
Q5W041

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MGKKIKKEVE PPPKDVFDPL MIESKKAATV VLMLNSPEEE ILAKACEAIY KFALKGEENK
61TTLLELGAVE PLTKLLTHED KIVRRNATMI FGILASNNDV KKLLRELDVM NSVIAQLAPE
121EEVVIHEFAS LCLANMSAEY TSKVQIFEHG GLEPLIRLLS SPDPDVKKNS MECIYNLVQD
181FQCRAKLQEL NAIPPILDLL KSEYPVIQLL ALKTLGVIAN DKESRTMLRD NQGLDHLIKI
241LETKELNDLH IEALAVIANC LEDMDTMVQI QQTGGLKKLL SFAENSTIPD IQKNAAKAIT
301KAAYDPENRK LFHEQEVEKC LVALLGSEND GTKIAASQAI SAMCENSGSK DFFNNQGIPQ
361LIQLLKSDNE EVREAAALAL ANLTTCNPAN ANAAAEADGI DPLINLLSSK RDGAIANAAT
421VLTNMAMQEP LRLNIQNHDI MHAIISPLRS ANTVVQSKAA LAVTATACDV EARTELRNSG
481GLEPLVELLR SKNDEVRKHA SWAVMVCAGD ELTANELCRL GALDILEEVN VSGTRKNKFS
541EAAYNKLLNN NLSLKYSQTG YLSSSNIIND GFYDYGRINP GTKLLPLKEL CLQEPSDLRA
601VLLINSKSYV SPPSSMEDKS DVGYGQSISS SSSLRRSSKE KNKKNSYHFS AGFGSPIEDK
661SEPASGRNTV LSKSATKEKG WRKSKGKKEE EKVKEEEEVM VVPKFVGEGS SDKEWCPPSD
721PDFSMYVYEV TKSILPITNI KEQIEDLAKY VAEKMGGKIP KEKLPDFSWE LHISELKFQL
781KSNVIPIGHV KKGIFYHRAL LFKALADRIG IGCSLVRGEY GRAWNEVMLQ NDSRKGVIGG
841LPAPEMYVID LMFHPGGLMK LRSREADLYR FI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

872 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.