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ARID5A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARID5A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARID5A antibodies →
$725.00each
Catalog Number: LS-G102743-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARID5A (NM_212481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARID5A | Modulator recognition factor 1 | MRF-1 | MRF1 | RFVG5814 | RP11-363D14 | Modulator recognition factor I
Origin Species
Human
NCBI GenBank
NM_212481
SwissProt
Q03989

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPVKGNRK QSTEGDALDP PASPKPAGKQ NGIQNPISLE DSPEAGGERE EEQEREEEQA
61FLVSLYKFMK ERHTPIERVP HLGFKQINLW KIYKAVEKLG AYELVTGRRL WKNVYDELGG
121SPGSTSAATC TRRHYERLVL PYVRHLKGED DKPLPTSKPR KQYKMAKENR GDDGATERPK
181KAKEERRMDQ MMPGKTKADA ADPAPLPSQE PPRNSTEQQG LASGSSVSFV GASGCPEAYK
241RLLSSFYCKG THGIMSPLAK KKLLAQVSKV EALQCQEEGC RHGAEPQASP AVHLPESPQS
301PKGLTENSRH RLTPQEGLQA PGGSLREEAQ AGPCPAAPIF KGCFYTHPTE VLKPVSQHPR
361DFFSRLKDGV LLGPPGKEGL SVKEPQLVWG GDANRPSAFH KGGSRKGILY PKPKACWVSP
421MAKVPAESPT LPPTFPSSPG LGSKRSLEEE GAAHSGKRLR AVSPFLKEAD AKKCGAKPAG
481SGLVSCLLGP ALGPVPPEAY RGTMLHCPLN FTGTPGPLKG QAALPFSPLV IPAFPAHFLA
541TAGPSPMAAG LMHFPPTSFD SALRHRLCPA SSAWHAPPVT TYAAPHFFHL NTKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

594 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.