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ARID3B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARID3B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARID3B antibodies →
$504.00each
Catalog Number: LS-G115287-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARID3B(ARID3B) (NM_006465), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bright-like protein | BDP | DRIL2 | ARID3B | Bright and dead ringer protein
Origin Species
Human
NCBI GenBank
NM_006465
SwissProt
Q8IVW6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPLQQQQQQ QQQQQKQPHL APLQMDAREK QGQQMREAQF LYAQKLVTQP TLLSATAGRP
61SGSTPLGPLA RVPPTAAVAQ VFERGNMNSE PEEEDGGLED EDGDDEVAEV AEKETQAASK
121YFHVQKVARQ DPRVAPMSNL LPAPGLPPHG QQAKEDHTKD ASKASPSVST AGQPNWNLDE
181QLKQNGGLAW SDDADGGRGR EISRDFAKLY ELDGDPERKE FLDDLFVFMQ KRGTPINRIP
241IMAKQILDLY MLYKLVTEKG GLVEIINKKI WREITKGLNL PTSITSAAFT LRTQYMKYLY
301AYECEKKALS SPAELQAAID GNRREGRRPS YSSSLFGYSP AAATAAAAAG APALLSPPKI
361RFPILGLGSS SGTNTSSPRI SPATTLRKGD GAPVTTVPVP NRLAVPVTLA SQQAGTRTAA
421LEQLRERLES GEPAEKKASR LSEEEQRLVQ QAFQRNFFSM ARQLPMKIRI NGREDRAEAS
481AAALNLTTSS IGSINMSVDI DGTTYAGVLF AQKPVVHLIT GSAPQSLGSS ASSSSSSHCS
541PSPTSSRGTP SAEPSTSWSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

560 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.