Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARID3A / DRIL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116094-100
Catalog Number: LS-G116094
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DRIL1(ARID3A) (NM_005224), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARID3A | ARID domain-containing 3A | BRIGHT | Dead ringer-like protein 1 | Dead ringer-like 1 | DRX | E2FBP1 | E2F-binding protein 1 | DRIL1 | DRIL3
- Origin Species
- Human
- NCBI GenBank
- NM_005224
- SwissProt
- Q99856
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLQAVMETL LQRQQRARQE LEARQQLPPD PPAAPHGRAR AAPDEDREPE SARMQRAQMA61ALAAMRAAAA GLGHPASPGG SEDGPPGSEE EDAAREGTPG SPGRGREGPG EEHFEDMASD121EDMKPKWEEE EMEEDLGEDE EEEEEDYEDE EEEEDEEGLG PPGPASLGTT ALFPRKAQPP181QAFRGDGVPR VLGGQERPGP GPAHPGGAAH VAPQLQPPDH GDWTYEEQFK QLYELDGDPK241RKEFLDDLFS FMQKRGTPVN RIPIMAKQVL DLFMLYVLVT EKGGLVEVIN KKLWREITKG301LNLPTSITSA AFTLRTQYME YLYPYECEKR GLSNPNELQA AIDSNRREGR RQSFGGSLFA361YSPGGAHGML SSPKLPVSSL GLAASTNGSS ITPAPKIKKE EDSAIPITVP GRLPVSLAGH421PVVAAQAAAV QAAAAQAAVA AQAAALEQLR EKLESAEPPE KKMALVADEQ QRLMQRALQQ481NFLAMAAQLP MSIRINSQAS ESRQDSAVNL TGTNGSNSIS MSVEINGIMY TGVLFAQPPA541PTPTSAPNKG GGGGGSSSSN AGGRGGNTGT SGGQAGPAGL STPSTSTSNN SLPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
593 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.