Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARID1B / BAF250B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111043-100
Catalog Number: LS-G111043
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARID1B (NM_020732), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 6A3-5 | ARID1B | BRG1-associated factor 250b | BRG1-binding protein ELD/OSA1 | DAN15 | ELD/OSA1 | ELD (eyelid)/OSA protein | KIAA1235 | MRD12 | HOsa2 | OSA2 | Osa homolog 2 | RP11-419L10.1 | BAF250B | BRG1-binding protein hELD/OSA1 | p250R
- Origin Species
- Human
- NCBI GenBank
- NM_020732
- SwissProt
- Q8NFD5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 232.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1METGLLPNHK LKTVGEAPAA PPHQQHHHHH HAHHHHHHAH HLHHHHALQQ QLNQFQQQQQ61QQQQQQQQQQ QQQHPISNNN SLGGAGGGAP QPGPDMEQPQ HGGAKDSAAG GQADPPGPPL121LSKPGDEDDA PPKMGEPAGG RYEHPGLGAL GTQQPPVAVP GGGGGPAAVP EFNNYYGSAA181PASGGPGGRA GPCFDQHGGQ QSPGMGMMHS ASAAAAGAPG SMDPLQNSHE GYPNSQCNHY241PGYSRPGAGG GGGGGGGGGG GSGGGGGGGG AGAGGAGAGA VAAAAAAAAA AAGGGGGGGY301GGSSAGYGVL SSPRQQGGGM MMGPGGGGAA SLSKAAAGSA AGGFQRFAGQ NQHPSGATPT361LNQLLTSPSP MMRSYGGSYP EYSSPSAPPP PPSQPQSQAA AAGAAAGGQQ AAAGMGLGKD421MGAQYAAASP AWAAAQQRSH PAMSPGTPGP TMGRSQGSPM DPMVMKRPQL YGMGSNPHSQ481PQQSSPYPGG SYGPPGPQRY PIGIQGRTPG AMAGMQYPQQ QDSGDATWKE TFWLMPPQYG541QQGVSGYCQQ GQQPYYSQQP QPPHLPPQAQ YLPSQSQQRY QPQQDMSQEG YGTRSQPPLA601PGKPNHEDLN LIQQERPSSL PDLSGSIDDL PTGTEATLSS AVSASGSTSS QGDQSNPAQS661PFSPHASPHL SSIPGGPSPS PVGSPVGSNQ SRSGPISPAS IPGSQMPPQP PGSQSESSSH721PALSQSPMPQ ERGFMAGTQR NPQMAQYGPQ QTGPSMSPHP SPGGQMHAGI SSFQQSNSSG781TYGPQMSQYG PQGNYSRPPA YSGVPSASYS GPGPGMGISA NNQMHGQGPS QPCGAVPLGR841MPSAGMQNRP FPGNMSSMTP SSPGMSQQGG PGMGPPMPTV NRKAQEAAAA VMQAAANSAQ901SRQGSFPGMN QSGLMASSSP YSQPMNNSSS LMNTQAPPYS MAPAMVNSSA ASVGLADMMS961PGESKLPLPL KADGKEEGTP QPESKSKKSS SSTTTGEKIT KVYELGNEPE RKLWVDRYLT1021FMEERGSPVS SLPAVGKKPL DLFRLYVCVK EIGGLAQVNK NKKWRELATN LNVGTSSSAA1081SSLKKQYIQY LFAFECKIER GEEPPPEVFS TGDTKKQPKL QPPSPANSGS LQGPQTPQST1141GSNSMAEVPG DLKPPTPAST PHGQMTPMQG GRSSTISVHD PFSDVSDSSF PKRNSMTPNA1201PYQQGMSMPD VMGRMPYEPN KDPFGGMRKV PGSSEPFMTQ GQMPNSSMQD MYNQSPSGAM1261SNLGMGQRQQ FPYGASYDRR HEPYGQQYPG QGPPSGQPPY GGHQPGLYPQ QPNYKRHMDG1321MYGPPAKRHE GDMYNMQYSS QQQEMYNQYG GSYSGPDRRP IQGQYPYPYS RERMQGPGQI1381QTHGIPPQMM GGPLQSSSSE GPQQNMWAAR NDMPYPYQNR QGPGGPTQAP PYPGMNRTDD1441MMVPDQRINH ESQWPSHVSQ RQPYMSSSAS MQPITRPPQP SYQTPPSLPN HISRAPSPAS1501FQRSLENRMS PSKSPFLPSM KMQKVMPTVP TSQVTGPPPQ PPPIRREITF PPGSVEASQP1561VLKQRRKITS KDIVTPEAWR VMMSLKSGLL AESTWALDTI NILLYDDSTV ATFNLSQLSG1621FLELLVEYFR KCLIDIFGIL MEYEVGDPSQ KALDHNAARK DDSQSLADDS GKEEEDAECI1681DDDEEDEEDE EEDSEKTESD EKSSIALTAP DAAADPKEKP KQASKFDKLP IKIVKKNNLF1741VVDRSDKLGR VQEFNSGLLH WQLGGGDTTE HIQTHFESKM EIPPRRRPPP PLSSAGRKKE1801QEGKGDSEEQ QEKSIIATID DVLSARPGAL PEDANPGPQT ESSKFPFGIQ QAKSHRNIKL1861LEDEPRSRDE TPLCTIAHWQ DSLAKRCICV SNIVRSLSFV PGNDAEMSKH PGLVLILGKL1921ILLHHEHPER KRAPQTYEKE EDEDKGVACS KDEWWWDCLE VLRDNTLVTL ANISGQLDLS1981AYTESICLPI LDGLLHWMVC PSAEAQDPFP TVGPNSVLSP QRLVLETLCK LSIQDNNVDL2041ILATPPFSRQ EKFYATLVRY VGDRKNPVCR EMSMALLSNL AQGDALAARA IAVQKGSIGN2101LISFLEDGVT MAQYQQSQHN LMHMQPPPLE PPSVDMMCRA AKALLAMARV DENRSEFLLH2161EGRLLDISIS AVLNSLVASV ICDVLFQIGQ LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2191 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.