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ARHGEF5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ARHGEF5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ARHGEF5 antibodies →
$725.00each
Catalog Number: LS-G115968-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF5 (NM_005435), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGEF5 | GEF5 | Ephexin-3 | Oncogene TIM | p60 | TIM1 | p60 TIM | TIM
Origin Species
Human
NCBI GenBank
NM_005435
SwissProt
Q12774

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
176.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEAEEAQRGA SPPISAIEEF SIIPEAPMRS SQVSALGLEA QEDEDPSYKW REEHRLSATQ
61QSELRDVCDY AIETMPSFPK EGSADVEPNQ ESLVAEACDT PEHWEAVPQS LAGRQARTLA
121PPELWACPIQ SEHLDMAPFS SDLGSEEEEV EFWPGLTSLT LGSGQAEEEE ETSSDNSGQT
181RYYSPCEEHP AETNQNEGSE SGTIRQGEEL PPEELQESQG LLHPQEVQVL EEQGQQEAGF
241RGEGTLREDV CADGLLGEEQ MIEQVNDEKG EQKQKQEQVQ DVMLGRQGER MGLTGEPEGL
301NDGEWEQEDM ERKAQGQGGP EQGEERKREL QVPEENRADS QDEKSQTFLG KSEEVTGKQE
361DHGIKEKGVP VSGQEAKEPE SWDGGRLGAV GRARSREEEN EHHGPSMPAL IAPEDSPHCD
421LFPGASYLMT QIPGTQTESR AEELSPAALS PSLEPIRCSH QPISLLGSFL TEESPDKEID
481QNSQQEESRL RKGTVSSQGT EVVFASASVT PPRTPDSAPP SPAEAYPITP ASVSARPPVA
541FPRRETSCAA RAPETASAPL SMDDPSPCGT SEMCPAALYG FPSTGTSPPR PPANSTGTVQ
601HLRSDSFPGS HRTEQTPDLV GMLLSYSHSE LPQRPPKPAI YSSVTPRRDR RSGRDYSTVS
661ASPTALSTLK QDSQESISNL ERPSSPPSIQ PWVSPHNPAF ATESPAYGSS PSFVSMEDVR
721IHEPLPPPPP QRRDTHPSVV ETDGHARVVV PTLKQHSHPP PLALGSGLHA PHKGPLPQAS
781DPAVARQHRP LPSTPDSSHH AQATPRWRYN KPLPPTPDLP QPHLPPISAP GSSRIYRPLP
841PLPIIDPPTE PPPLPPKSRG RSRSTRGGHM NSGGHAKTRP ACQDWTVPLP ASAGRTSWPP
901ATARSTESFT STSRSKSEVS PGMAFSNMTN FLCPSSPTTP WTPELQGPTS KDEAGVSEHP
961EAPAREPLRR TTPQQGASGP GRSPVGQARQ PEKPSHLHLE KASSWPHRRD SGRPPGDSSG
1021QAVAPSEGAN KHKGWSRQGL RRPSILPEGS SDSRGPAVEK HPGPSDTVVF REKKPKEVMG
1081GFSRRCSKLI NSSQLLYQEY SDVVLNKEIQ SQQRLESLSE TPGPSSPRQP RKALVSSESY
1141LQRLSMASSG SLWQEIPVVR NSTVLLSMTH EDQKLQEVKF ELIVSEASYL RSLNIAVDHF
1201QLSTSLRATL SNQEHQWLFS RLQDVRDVSA TFLSDLEENF ENNIFSFQVC DVVLNHAPDF
1261RRVYLPYVTN QTYQERTFQS LMNSNSNFRE VLEKLESDPV CQRLSLKSFL ILPFQRITRL
1321KLLLQNILKR TQPGSSEEAE ATKAHHALEQ LIRDCNNNVQ SMRRTEELIY LSQKIEFECK
1381IFPLISQSRW LVKSGELTAL EFSASPGLRR KLNTRPVHLH LFNDCLLLSR PREGSRFLVF
1441DHAPFSSIRG EKCEMKLHGP HKNLFRLFLR QNTQGAQAEF LFRTETQSEK LRWISALAMP
1501REELDLLECY NSPQVQCLRA YKPRENDELA LEKADVVMVT QQSSDGWLEG VRLSDGERGW
1561FPVQQVEFIS NPEVRAQNLK EAHRVKTAKL QLVEQQA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1597 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.