Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGEF40 / SOLO Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112012-100
Catalog Number: LS-G112012
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FLJ10357(ARHGEF40) (NM_018071), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Protein Solo | SOLO | FLJ10357
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 164.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPEPVEDCV QSTLAALYPP FEATAPTLLG QVFQVVERTY REDALRYTLD FLVPAKHLLA61KVQQEACAQY SGFLFFHEGW PLCLHEQVVV QLAALPWQLL RPGDFYLQVV PSAAQAPRLA121LKCLAPGGGR VQEVPVPNEA CAYLFTPEWL QGINKDRPTG RLSTCLLSAP SGIQRLPWAE181LICPRFVHKE GLMVGHQPST LPPELPSGPP GLPSPPLPEE ALGTRSPGDG HNAPVEGPEG241EYVELLEVTL PVRGSPTDAE GSPGLSRVRT VPTRKGAGGK GRHRRHRAWM HQKGLGPRGQ301DGARPPGEGS STGASPESPP GAEAVPEAAV LEVSEPPAEA VGEASGSCPL RPGELRGGGG361GGQGAEGPPG TPRRTGKGNR RKKRAAGRGA LSRGGDSAPL SPGDKEDASH QEALGNLPSP421SEHKLPECHL VKEEYEGSGK PESEPKELKT AGEKEPQLSE ACGPTEEGAG ERELEGPGLL481CMAGHTGPEG PLSDTPTPPL ETVQEGKGDN IPEEALAVSV SDHPDVAWDL MASGFLILTG541GVDQSGRALL TITPPCPPEE PPPSRDTLNT TLHYLHSLLR PDLQTLGLSV LLDLRQAPPL601PPALIPALSQ LQDSGDPPLV QRLLILIHDD LPTELCGFQG AEVLSENDLK RVAKPEELQW661ELGGHRDPSP SHWVEIHQEV VRLCRLCQGV LGSVRQAIEE LEGAAEPEEE EAVGMPKPLQ721KVLADPRLTA LQRDGGAILM RLRSTPSSKL EGQGPATLYQ EVDEAIHQLV RLSNLHVQQQ781EQRQCLRRLQ QVLQWLSGPG EEQLASFAMP GDTLSALQET ELRFRAFSAE VQERLAQARE841ALALEENATS QKVLDIFEQR LEQVESGLHR ALRLQRFFQQ AHEWVDEGFA RLAGAGPGRE901AVLAALALRR APEPSAGTFQ EMRALALDLG SPAALREWGR CQARCQELER RIQQHVGEEA961SPRGYRRRRA DGASSGGAQW GPRSPSPSLS SLLLPSSPGP RPAPSHCSLA PCGEDYEEEG1021PELAPEAEGR PPRAVLIRGL EVTSTEVVDR TCSPREHVLL GRARGPDGPW GVGTPRMERK1081RSISAQQRLV SELIACEQDY VATLSEPVPP PGPELTPELR GTWAAALSAR ERLRSFHRTH1141FLRELQGCAT HPLRIGACFL RHGDQFSLYA QYVKHRHKLE NGLAALSPLS KGSMEAGPYL1201PRALQQPLEQ LTRYGRLLEE LLREAGPELS SECRALGAAV QLLREQEARG RDLLAVEAVR1261GCEIDLKEQG QLLHRDPFTV ICGRKKCLRH VFLFEHLLLF SKLKGPEGGS EMFVYKQAFK1321TADMGLTENI GDSGLCFELW FRRRRAREAY TLQATSPEIK LKWTSSIAQL LWRQAAHNKE1381LRVQQMVSMG IGNKPFLDIK ALGERTLSAL LTGRAARTRA SVAVSSFEHA GPSLPGLSPG1441ACSLPARVEE EAWDLDVKQI SLAPETLDSS GDVSPGPRNS PSLQPPHPGS STPTLASRGI1501LGLSRQSHAR ALSDPTTPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1519 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.